Nucleoprotein (396-404) (TFA) (Synonyms: NP 396 TFA)

Name: Nucleoprotein (396-404) (TFA)
Brand: MedChemExpress
SKU: HY-P1571A
Rating: 4.5 (1 reviews)

Cat. No.: HY-P1571A Purity: 95.24%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Nucleoprotein (396-404) TFA is the 396 to 404 fragment of lymphocytic choriomeningitis virus (LCMV). Nucleoprotein (396-404) TFA is the H-2D(b)-restricted immunodominant epitope and can be used as a molecular model of viral antigen .

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Nucleoprotein (396-404) (TFA) Chemical Structure

Size	Price	Stock	Quantity
1 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 140	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 210	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Nucleoprotein (396-404) (TFA):

Nucleoprotein (396-404) Get quote

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Description

Molecular Weight

1192.20

Formula

C₅₂H₇₂F₃N₁₃O₁₆

Appearance

Solid

Color

White to off-white

Sequence

Phe-Gln-Pro-Gln-Asn-Gly-Gln-Phe-Ile

Sequence Shortening

FQPQNGQFI

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (83.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.8388 mL	4.1939 mL	8.3879 mL
5 mM	0.1678 mL	0.8388 mL	1.6776 mL
10 mM	0.0839 mL	0.4194 mL	0.8388 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 95.24%

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (255 KB) LCMS (119 KB) Elemental Analysis Report (115 KB)

Handling Instructions (2659 KB)

References

[1]. Gairin JE, et al. Optimal lymphocytic choriomeningitis virus sequences restricted by H-2Db major histocompatibility complex class I molecules and presented to cytotoxic T lymphocytes. J Virol. 1995 Apr;69(4):2297-305. [Content Brief]

[2]. Hudrisier D, et al. Structural and functional identification of major histocompatibility complex class I-restricted self-peptides as naturally occurring molecular mimics of viral antigens. Possible role in CD8+ T cell-mediated, virus-induced autoimmune di [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.8388 mL	4.1939 mL	8.3879 mL	20.9696 mL
	5 mM	0.1678 mL	0.8388 mL	1.6776 mL	4.1939 mL
	10 mM	0.0839 mL	0.4194 mL	0.8388 mL	2.0970 mL
	15 mM	0.0559 mL	0.2796 mL	0.5592 mL	1.3980 mL
	20 mM	0.0419 mL	0.2097 mL	0.4194 mL	1.0485 mL
	25 mM	0.0336 mL	0.1678 mL	0.3355 mL	0.8388 mL
	30 mM	0.0280 mL	0.1398 mL	0.2796 mL	0.6990 mL
	40 mM	0.0210 mL	0.1048 mL	0.2097 mL	0.5242 mL
	50 mM	0.0168 mL	0.0839 mL	0.1678 mL	0.4194 mL
	60 mM	0.0140 mL	0.0699 mL	0.1398 mL	0.3495 mL
	80 mM	0.0105 mL	0.0524 mL	0.1048 mL	0.2621 mL

Nucleoprotein (396-404) (TFA) Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nucleoprotein (396-404) (TFA) (Synonyms: NP 396 TFA)

Customer Review

Other Forms of Nucleoprotein (396-404) (TFA):

Nucleoprotein (396-404) (TFA) Related Antibodies

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•Related Screening Libraries:

•Related Small Molecules:

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Biological Activity

Purity & Documentation

References

Customer Review

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Complete Stock Solution Preparation Table

Nucleoprotein (396-404) (TFA) Related Classifications

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