Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride

Name: Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride
Brand: MedChemExpress
SKU: HY-137529
Rating: 4.5 (1 reviews)

Cat. No.: HY-137529 Purity: 99.92%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Nα-Benzoyl-L-arginine 4-nitroanilide (hydrochloride) is aarginine derivatives.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride Chemical Structure

CAS No. : 21653-40-7

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 33	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 33	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 45	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Purity & Documentation
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Description

Nα-Benzoyl-L-arginine 4-nitroanilide (hydrochloride) is aarginine derivatives.

Molecular Weight

434.88

Formula

C₁₉H₂₃ClN₆O₄

CAS No.

21653-40-7

Appearance

Solid

Color

White to light yellow

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (229.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 5 mg/mL (11.50 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2995 mL	11.4974 mL	22.9948 mL
5 mM	0.4599 mL	2.2995 mL	4.5990 mL
10 mM	0.2299 mL	1.1497 mL	2.2995 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (244 KB) SDS (393 KB)

COA (250 KB) HNMR (303 KB) LCMS (107 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.2995 mL	11.4974 mL	22.9948 mL	57.4871 mL
	5 mM	0.4599 mL	2.2995 mL	4.5990 mL	11.4974 mL
	10 mM	0.2299 mL	1.1497 mL	2.2995 mL	5.7487 mL
DMSO	15 mM	0.1533 mL	0.7665 mL	1.5330 mL	3.8325 mL
	20 mM	0.1150 mL	0.5749 mL	1.1497 mL	2.8744 mL
	25 mM	0.0920 mL	0.4599 mL	0.9198 mL	2.2995 mL
	30 mM	0.0766 mL	0.3832 mL	0.7665 mL	1.9162 mL
	40 mM	0.0575 mL	0.2874 mL	0.5749 mL	1.4372 mL
	50 mM	0.0460 mL	0.2299 mL	0.4599 mL	1.1497 mL
	60 mM	0.0383 mL	0.1916 mL	0.3832 mL	0.9581 mL
	80 mM	0.0287 mL	0.1437 mL	0.2874 mL	0.7186 mL
	100 mM	0.0230 mL	0.1150 mL	0.2299 mL	0.5749 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.