2. GPCR/G Protein Apoptosis
  3. GPR68 Ferroptosis
  4. Ogremorphin

Ogremorphin  (Synonyms: OGM; GPR68-IN-1)

Cat. No.: HY-154954 Purity: 99.65%
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Ogremorphin (OGM) is a G protein coupled sensor GPR68 inhibitor with anti-inflammatory and anti-tumor activities. Ogremorphin can inhibit the migration of human melanoma cells and induce ferroptosis in glioblastoma cells.

For research use only. We do not sell to patients.

Ogremorphin Chemical Structure

Ogremorphin Chemical Structure

CAS No. : 352563-21-4

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1 mg USD 230 In-stock
5 mg USD 590 In-stock
10 mg USD 950 In-stock
25 mg USD 1900 In-stock
50 mg USD 3080 In-stock
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Based on 1 publication(s) in Google Scholar

Ogremorphin Related Antibodies

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Description

Ogremorphin (OGM) is a G protein coupled sensor GPR68 inhibitor with anti-inflammatory and anti-tumor activities. Ogremorphin can inhibit the migration of human melanoma cells and induce ferroptosis in glioblastoma cells[1][2][3].
IC50 & Target

GPR68[1].
In Vitro

Ogremorphin (1-5 µM) can reduce the release of inflammatory factors induced by lipopolysaccharide or acidosis in human pulmonary artery endothelial cells, and inhibit inflammatory responses[1].
Ogremorphin (5 µM, 5 days) can inhibit the migration of human melanoma WM-115 cells[2].
Ogremorphin (0-100 µM, 72 h) can induce ferroptosis and inhibit tumor cell viability in glioblastoma U87 cells by promoting the expression of iron death related markers and mediators[3].
Ogremorphin (0-100 µM) can reduce the survival rate of patient derived cell lines (PDX and neurospheres), with LC50 value of 0.42-2.7 µM[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ogremorphin Related Antibodies

Cell Viability Assay[3].

Cell Line: U87 and U138 cell lines
Concentration: 0-100 µM
Incubation Time: 72 h
Result: Reduced the vitality of glioblastoma cells.

RT-PCR[1].

Cell Line: Human pulmonary arterial endothelial cells (HPAECs)
Concentration: 1-5 µM
Incubation Time:
Result: Reduced the upregulation of inflammatory cytokine/chemokine genes TNF - α, ICAM-1, VCAM-1, IL-6, IL-8, IL-1 β, and CXCL5.

Cell Migration Assay [2].

Cell Line: WM115, A2050 and MEWO cell lines
Concentration: 5 µM
Incubation Time: 5 days
Result: Inhibited migration in WM115, A2050 and MEWO cell lines.
In Vivo

Ogremorphin can alleviate vascular leakage, pulmonary inflammation, and pulmonary endothelial dysfunction caused by intratracheal injection of LPS or acidosis in mice[1].
Ogremorphin (10 μM) can cause abnormal melanocyte pigmentation and developmental disorders in zebrafish embryos[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Zebrafish[3].
Dosage: 10 μM
Administration: Added to a 96 well microtiter plate (5 embryos/well) arranged with zebrafish fertilized eggs
Result: Induced phenotypes encompassing a wavy notochord, abnormal pigmentation, craniofacial defects, ventral curvature, and a shortened body axis.
Molecular Weight

359.44

Formula

C21H17N3OS
CAS No.
Appearance

Solid

Color

Yellow to orange

SMILES

O=C1NC2=CC=C(CC)C=C2C13SC(C4=C5C=CC=CC5=CC=C4)=NN3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (139.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7821 mL 13.9105 mL 27.8211 mL
5 mM 0.5564 mL 2.7821 mL 5.5642 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 1.25 mg/mL (3.48 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.65%

COA (257 KB) HNMR (144 KB) RP-HPLC (284 KB) LCMS (379 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7821 mL 13.9105 mL 27.8211 mL 69.5526 mL
5 mM 0.5564 mL 2.7821 mL 5.5642 mL 13.9105 mL
10 mM 0.2782 mL 1.3911 mL 2.7821 mL 6.9553 mL
15 mM 0.1855 mL 0.9274 mL 1.8547 mL 4.6368 mL
20 mM 0.1391 mL 0.6955 mL 1.3911 mL 3.4776 mL
25 mM 0.1113 mL 0.5564 mL 1.1128 mL 2.7821 mL
30 mM 0.0927 mL 0.4637 mL 0.9274 mL 2.3184 mL
40 mM 0.0696 mL 0.3478 mL 0.6955 mL 1.7388 mL
50 mM 0.0556 mL 0.2782 mL 0.5564 mL 1.3911 mL
60 mM 0.0464 mL 0.2318 mL 0.4637 mL 1.1592 mL
80 mM 0.0348 mL 0.1739 mL 0.3478 mL 0.8694 mL
100 mM 0.0278 mL 0.1391 mL 0.2782 mL 0.6955 mL
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Ogremorphin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ogremorphin352563-21-4OGM GPR68-IN-1GPR68FerroptosisG Protein-Coupled Receptor 68G-protein coupled sensormelanoma cellsglioblastomaInhibitorinhibitorinhibit

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