2. Membrane Transporter/Ion Channel
  3. Sodium Channel
  4. Oxybuprocaine hydrochloride

Oxybuprocaine hydrochloride  (Synonyms: Benoxinate hydrochloride)

Cat. No.: HY-B1288 Purity: 99.49%
SDS COA Handling Instructions

Molarity Calculator

Oxybuprocaine hydrochloride (Benoxinate hydrochloride) reversibly blocks sodium channels and prevents propagation of painful nerve impulses in the cornea, conjunctiva, and sclera. Oxybuprocaine hydrochloride is used especially in ophthalmology and otolaryngology.

For research use only. We do not sell to patients.

Oxybuprocaine hydrochloride Chemical Structure

Oxybuprocaine hydrochloride Chemical Structure

CAS No. : 5987-82-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 33 In-stock
Solution
10 mM * 1 mL in DMSO USD 33 In-stock
Solid
5 mg USD 30 In-stock
10 mg USD 45 In-stock
50 mg USD 75 In-stock
100 mg USD 115 In-stock
250 mg USD 210 In-stock
500 mg   Get quote  
1 g   Get quote  

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Based on 1 publication(s) in Google Scholar

Other Forms of Oxybuprocaine hydrochloride:

Oxybuprocaine hydrochloride Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Oxybuprocaine hydrochloride (Benoxinate hydrochloride) reversibly blocks sodium channels and prevents propagation of painful nerve impulses in the cornea, conjunctiva, and sclera. Oxybuprocaine hydrochloride is used especially in ophthalmology and otolaryngology[1][2][3].

In Vivo

Oxybuprocaine hydrochloride (Benoxinate hydrochloride; 27.6, 51.7, 103.5, 413.9 μg; s.c.) provides a dose-dependent block to pinpricks in rats[1].
Oxybuprocaine hydrochloride (27.6 μg) produces 100% of sensory/nociceptive block[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats (203-253 g)[1]
Dosage: 27.6, 51.7, 103.5, 413.9 μg
Administration: SC
Result: Provided a dose-dependent block to pinpricks.
Clinical Trial
Molecular Weight

344.88

Formula

C17H29ClN2O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(OCCN(CC)CC)C1=CC=C(N)C(OCCCC)=C1.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (289.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : ≥ 100 mg/mL (289.96 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8996 mL 14.4978 mL 28.9956 mL
5 mM 0.5799 mL 2.8996 mL 5.7991 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.25 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.25 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.49%

SDS (393 KB)

COA (244 KB) HNMR (256 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.8996 mL 14.4978 mL 28.9956 mL 72.4890 mL
5 mM 0.5799 mL 2.8996 mL 5.7991 mL 14.4978 mL
10 mM 0.2900 mL 1.4498 mL 2.8996 mL 7.2489 mL
15 mM 0.1933 mL 0.9665 mL 1.9330 mL 4.8326 mL
20 mM 0.1450 mL 0.7249 mL 1.4498 mL 3.6244 mL
25 mM 0.1160 mL 0.5799 mL 1.1598 mL 2.8996 mL
30 mM 0.0967 mL 0.4833 mL 0.9665 mL 2.4163 mL
40 mM 0.0725 mL 0.3624 mL 0.7249 mL 1.8122 mL
50 mM 0.0580 mL 0.2900 mL 0.5799 mL 1.4498 mL
60 mM 0.0483 mL 0.2416 mL 0.4833 mL 1.2081 mL
80 mM 0.0362 mL 0.1812 mL 0.3624 mL 0.9061 mL
100 mM 0.0290 mL 0.1450 mL 0.2900 mL 0.7249 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Oxybuprocaine hydrochloride Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Oxybuprocaine5987-82-6BenoxinateSodium ChannelNa channelsNa+ channelspropagationpainfulnerveimpulsescorneaconjunctivascleraophthalmologyotolaryngologyALOParkinson’sdiseaseInhibitorinhibitorinhibit

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