1. Cell Cycle/DNA Damage NF-κB Apoptosis
  2. PERK Keap1-Nrf2 Apoptosis
  3. Pachypodol

Pachypodol is an orally active methoxyflavonoid compound. Pachypodol activates the ERK-dependent Nrf2 pathway and inhibits Apoptosis. Pachypodol exhibits activities such as antioxidant, cytoprotective, anti-inflammatory effects. Pachypodol improves cognition. Pachypodol exerts protective effects against cardiac and liver damage. Pachypodol has anticancer activity against colon cancer.

For research use only. We do not sell to patients.

Pachypodol Chemical Structure

Pachypodol Chemical Structure

CAS No. : 33708-72-4

Size Price Stock Quantity
1 mg In-stock
5 mg In-stock
10 mg In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Pachypodol:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Pachypodol is an orally active methoxyflavonoid compound. Pachypodol activates the ERK-dependent Nrf2 pathway and inhibits Apoptosis. Pachypodol exhibits activities such as antioxidant, cytoprotective, anti-inflammatory effects. Pachypodol improves cognition. Pachypodol exerts protective effects against cardiac and liver damage. Pachypodol has anticancer activity against colon cancer[1][2][3][4][5].

Cellular Effect
Cell Line Type Value Description References
KB IC50
> 47.8 μM
Compound: 7
Cytotoxicity against human KB cells by sulforhodamine B assay
Cytotoxicity against human KB cells by sulforhodamine B assay
[PMID: 26928423]
NCI-H460 IC50
17.2 μM
Compound: 10
Inhibition of ABCG2 expressed in human NCI-H460 cells assessed as inhibition of PhA accumulation after 2 to 20 hrs relative to fumitremorgin C
Inhibition of ABCG2 expressed in human NCI-H460 cells assessed as inhibition of PhA accumulation after 2 to 20 hrs relative to fumitremorgin C
[PMID: 21275386]
Vero IC50
> 47.8 μM
Compound: 7
Cytotoxicity against African green monkey Vero cells by sulforhodamine B assay
Cytotoxicity against African green monkey Vero cells by sulforhodamine B assay
[PMID: 26928423]
In Vitro

Pachypodol (3-30 μM; 1-12 h) significantly prevents oxidative stress-induced cell death in HepG2 cells in a concentration-dependent manner[1].
Pachypodol (0-30 μM; 24 h) inhibits the growth of CaCo-2 colon cancer cell line, with an IC50 value of 185.6 μM[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: HepG2 cells
Concentration: 30 μM
Incubation Time: 10 min, 30 min, 1 h, 3 h, 6 h, 12 h, 24 h
Result: Upregulated the protein levels of Nrf2-controlled antioxidant genes such as GCLC and NQO1.
Increased ERK phosphorylation.
In Vivo

Pachypodol (10 mg/kg; 30 days) attenuates Arsenic triggered cardiac damage in rats via rectifying oxidative stress, inflammation, apoptosis and histopathological alterations[3].
Pachypodol (10-30 mg/kg; i.v.; 30 min before isoflurane exposure) reduces neuronal apoptosis and oxidative stress in Isoflurane (HY-A0134)-induced neuron-injured rats by regulating the JNK/ERK pathway, and also ameliorates cognitive function and neurological scores[4].
Pachypodol (20 mg/kg; p.o.; once a day; 7-28 days) significantly alleviates Cisplatin (HY-17394)-induced hepatic impairment in albino rats by inhibiting oxidative stress, inflammation, and apoptosis, and mitigates histopathological liver damage[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Seven-day-old Sprague-Dawley rat pups (15-18 g), Isoflurane (HY-A0134)-induced neuronal injury model in developing brain[4]
Dosage: 10 mg/kg, 30 mg/kg
Administration: Intravenous injection, once (30 min before Isoflurane exposure)
Result: Reduced the levels of inflammatory mediators such as TNF-α, IL-6, and IL-1β in the serum.
Reduced oxidative stress in the brain tissue, as shown by decreased ROS and MDA levels and increased iNOS activity and protein expression.
Ameliorated neuronal cell apoptosis, as indicated by the reduced expression of Bax and caspase-3 and increased expression of Bcl-2, and decreased the number of TUNEL-positive cells in the hippocampus.
Regulated the JNK/ERK/Akt pathway, reducing the expression of p-JNK/JNK and p-ERK/ERK and increasing the expression of p-Akt/Akt.
Improved cognitive function, as shown by increased time spent in the target quadrant and number of crossings and decreased escape latency, and reduced the neurological score.
Molecular Weight

344.32

Formula

C18H16O7

CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O=C1C(OC)=C(C2=CC(OC)=C(O)C=C2)OC3=CC(OC)=CC(O)=C13

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (290.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9043 mL 14.5214 mL 29.0428 mL
5 mM 0.5809 mL 2.9043 mL 5.8085 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.82%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9043 mL 14.5214 mL 29.0428 mL 72.6069 mL
5 mM 0.5809 mL 2.9043 mL 5.8086 mL 14.5214 mL
10 mM 0.2904 mL 1.4521 mL 2.9043 mL 7.2607 mL
15 mM 0.1936 mL 0.9681 mL 1.9362 mL 4.8405 mL
20 mM 0.1452 mL 0.7261 mL 1.4521 mL 3.6303 mL
25 mM 0.1162 mL 0.5809 mL 1.1617 mL 2.9043 mL
30 mM 0.0968 mL 0.4840 mL 0.9681 mL 2.4202 mL
40 mM 0.0726 mL 0.3630 mL 0.7261 mL 1.8152 mL
50 mM 0.0581 mL 0.2904 mL 0.5809 mL 1.4521 mL
60 mM 0.0484 mL 0.2420 mL 0.4840 mL 1.2101 mL
80 mM 0.0363 mL 0.1815 mL 0.3630 mL 0.9076 mL
100 mM 0.0290 mL 0.1452 mL 0.2904 mL 0.7261 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Pachypodol
Cat. No.:
HY-N3121
Quantity:
MCE Japan Authorized Agent: