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  3. Fluorescent Dye
  4. PFB-FDG

PFB-FDG is a non-fluorescent galactosidase substrate that can be hydrolysed to green fluorescent PFB-F (Ex=485 nm, Em=535 nm). PFB-FDG can be used for the determination of β-galactosidase activity.

For research use only. We do not sell to patients.

PFB-FDG Chemical Structure

PFB-FDG Chemical Structure

CAS No. : 209540-64-7

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Based on 1 publication(s) in Google Scholar

PFB-FDG Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

PFB-FDG is a non-fluorescent galactosidase substrate that can be hydrolysed to green fluorescent PFB-F (Ex=485 nm, Em=535 nm). PFB-FDG can be used for the determination of β-galactosidase activity[1].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Measurement of β-galactosidase activity[1]:
1. Incubate 2 × 105 cells with 50 μg/mL of PFB-FDG in serum-free DMEM containing 25 mM Hepes (pH 7.4) for 2 h at 37℃. (Mononuclear cells for example, different cell concentrations vary)
2. Wash cells once with ice-cold PBS.
3. Analyze cells by flow cytometry (Ex=485 nm, Em=535 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PFB-FDG Related Antibodies
Molecular Weight

865.66

Formula

C39H32F5NO16
CAS No.
Appearance

Solid

Color

Light yellow to yellow

Emission (Em)

535
Excitation (Ex)

488
SMILES

O[C@H]([C@H]1O)[C@@H](O)[C@@H](CO)O[C@H]1OC(C=C2)=CC3=C2C4(OC(C5=C4C=CC(NC(C6=C(F)C(F)=C(F)C(F)=C6F)=O)=C5)=O)C7=C(O3)C=C(O[C@@H]8O[C@H](CO)[C@H](O)[C@H](O)[C@H]8O)C=C7
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (115.52 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1552 mL 5.7759 mL 11.5519 mL
5 mM 0.2310 mL 1.1552 mL 2.3104 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1552 mL 5.7759 mL 11.5519 mL 28.8797 mL
5 mM 0.2310 mL 1.1552 mL 2.3104 mL 5.7759 mL
10 mM 0.1155 mL 0.5776 mL 1.1552 mL 2.8880 mL
15 mM 0.0770 mL 0.3851 mL 0.7701 mL 1.9253 mL
20 mM 0.0578 mL 0.2888 mL 0.5776 mL 1.4440 mL
25 mM 0.0462 mL 0.2310 mL 0.4621 mL 1.1552 mL
30 mM 0.0385 mL 0.1925 mL 0.3851 mL 0.9627 mL
40 mM 0.0289 mL 0.1444 mL 0.2888 mL 0.7220 mL
50 mM 0.0231 mL 0.1155 mL 0.2310 mL 0.5776 mL
60 mM 0.0193 mL 0.0963 mL 0.1925 mL 0.4813 mL
80 mM 0.0144 mL 0.0722 mL 0.1444 mL 0.3610 mL
100 mM 0.0116 mL 0.0578 mL 0.1155 mL 0.2888 mL
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PFB-FDG Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PFB-FDG209540-64-7Fluorescent Dyeβ-galactosidasefluorescent dyesInhibitorinhibitorinhibit

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HY-D1699
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