2. Metabolic Enzyme/Protease Anti-infection
  3. Dihydroorotate Dehydrogenase Parasite
  4. PfDHODH-IN-2

PfDHODH-IN-2, a dihydrothiophenone derivative (Compound 11), is a potent Plasmodium falciparum dihydroorotate dehydrogenase (PfDHODH) inhibitor with an IC50 of 1.11 µM. PfDHODH-IN-2 acts as an antimalarial agent and can be used for the research of malaria.

For research use only. We do not sell to patients.

PfDHODH-IN-2 Chemical Structure

PfDHODH-IN-2 Chemical Structure

CAS No. : 425629-94-3

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10 mM * 1 mL in DMSO
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10 mM * 1 mL in DMSO USD 197 In-stock
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Based on 1 publication(s) in Google Scholar

PfDHODH-IN-2 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

PfDHODH-IN-2, a dihydrothiophenone derivative (Compound 11), is a potent Plasmodium falciparum dihydroorotate dehydrogenase (PfDHODH) inhibitor with an IC50 of 1.11 µM. PfDHODH-IN-2 acts as an antimalarial agent and can be used for the research of malaria[1].

IC50 & Target

IC50: 1.113 µM (PfDHODH)
IC50: >50 µM ( hDHODH)[1]
In Vitro

Malaria is a global parasitic infectious disease caused by Plasmodium parasites, among which Plasmodium falciparum is the most dangerous one with the highest rates of complications and mortality[1].
PfDHODH-IN-2 shows an overall selectivity for PfDHODH (IC50=1.113 µM) over hDHODH (IC50>50 µM). PfDHODH-IN-2 demonstrats in vitro potency against the 3D7 and Dd2 strains of P. falciparum with IC50 values of both >20 µM, respectively in whole cell assays[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PfDHODH-IN-2 Related Antibodies
Molecular Weight

297.76

Formula

C13H12ClNO3S
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(C1=C(NC2=CC=C(Cl)C=C2)SCC1=O)OCC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 12.5 mg/mL (41.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3584 mL 16.7920 mL 33.5841 mL
5 mM 0.6717 mL 3.3584 mL 6.7168 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 1.25 mg/mL (4.20 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 1.25 mg/mL (4.20 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3584 mL 16.7920 mL 33.5841 mL 83.9602 mL
5 mM 0.6717 mL 3.3584 mL 6.7168 mL 16.7920 mL
10 mM 0.3358 mL 1.6792 mL 3.3584 mL 8.3960 mL
15 mM 0.2239 mL 1.1195 mL 2.2389 mL 5.5973 mL
20 mM 0.1679 mL 0.8396 mL 1.6792 mL 4.1980 mL
25 mM 0.1343 mL 0.6717 mL 1.3434 mL 3.3584 mL
30 mM 0.1119 mL 0.5597 mL 1.1195 mL 2.7987 mL
40 mM 0.0840 mL 0.4198 mL 0.8396 mL 2.0990 mL
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PfDHODH-IN-2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PfDHODH-IN-2425629-94-3Dihydroorotate DehydrogenaseParasiteDHODHPlasmodium falciparumPfDHODH3D7malariaparasitedihydrothiophenonederivativeInhibitorinhibitorinhibit

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