2. Apoptosis Autophagy NF-κB MAPK/ERK Pathway PI3K/Akt/mTOR Stem Cell/Wnt JAK/STAT Signaling Protein Tyrosine Kinase/RTK Epigenetics
  3. Apoptosis Autophagy NF-κB p38 MAPK PI3K Akt mTOR STAT JAK
  4. Physalin A

Physalin A is a biologically active withanolide. Physalin A shows anti-inflammatory, antifibrotic and ameliorative effects on autophagy in models of disc degeneration. Physalin A has antitumor activity and can induce apoptosis, ROS production and G2/M phase cell cycle arrest. Besides. Physalin A can significantly increase the activity of quinone reductase and increase the expression of detoxifying enzymesc.

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Physalin A Chemical Structure

Physalin A Chemical Structure

CAS No. : 23027-91-0

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Based on 1 publication(s) in Google Scholar

Physalin A Related Antibodies

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Description

Physalin A is a biologically active withanolide. Physalin A shows anti-inflammatory, antifibrotic and ameliorative effects on autophagy in models of disc degeneration. Physalin A has antitumor activity and can induce apoptosis, ROS production and G2/M phase cell cycle arrest. Besides. Physalin A can significantly increase the activity of quinone reductase and increase the expression of detoxifying enzymesc[1][2][3][4].

Cellular Effect
Cell Line Type Value Description References
HeLa IC50
> 50 μM
Compound: PA-2
Inhibition of TNFalpha-induced IkappaBalpha (unknown origin) phosphorylation expressed in human HeLa cells pretreated 30 mins before TNFalpha addition measured up to 120 mins by ELISA in presence of proteasome inhibitor MG-132
Inhibition of TNFalpha-induced IkappaBalpha (unknown origin) phosphorylation expressed in human HeLa cells pretreated 30 mins before TNFalpha addition measured up to 120 mins by ELISA in presence of proteasome inhibitor MG-132
[PMID: 24900739]
HeLa IC50
22.7 μM
Compound: PA-2
Inhibition of TNFalpha-induced NF-kappaB activation (unknown origin) expressed in human HeLa cells pretreated 30 mins before TNFalpha addition after 7 hrs by luciferase reporter gene assay
Inhibition of TNFalpha-induced NF-kappaB activation (unknown origin) expressed in human HeLa cells pretreated 30 mins before TNFalpha addition after 7 hrs by luciferase reporter gene assay
[PMID: 24900739]
HT-1080 IC50
10.7 μM
Compound: 1
Cytotoxicity against human HT1080 cells assessed as growth inhibition after 24 hrs by MTT assay
Cytotoxicity against human HT1080 cells assessed as growth inhibition after 24 hrs by MTT assay
[PMID: 23647462]
In Vitro

Physalin A (28.4 μM; 0-24 h) induces ROS production, G2/M phase cell cycle arrest and triggeres phosphorylation of P38 and ERK in A549 cells[2].
Physalin A (0-15 μM; 24 h) inhibits the cell viability and induces cell apoptosis in human non-small cell lung cancer[3].
Physalin A (20 μM; 48 h) improves TGF-β1-induced fibrosis in NP cells[4].
Physalin A (20 μM; 48 h) reduces inflammation by inhibiting NF-κB and MAPK signaling pathways and enhances autophagy by blocking PI3K/AKT/mTOR signaling pathways in IL-1β-treated NP cells[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Physalin A Related Antibodies

Western Blot Analysis[2]

Cell Line: A549 cells
Concentration: 28.4 μM
Incubation Time: 6, 12 and 24 h
Result: Increased the phosphorylation levels P38 and ERK in a time-dependent manner.

Western Blot Analysis[3]

Cell Line: H292, H358 and H1975 cells
Concentration: 0, 2.5, 5, 10 and 15 μM
Incubation Time: 24 h
Result: Increased the levels of apoptotic markers, such as cleaved poly(ADP-ribose) polymerase (PARP), cleaved caspase-3 and cleaved caspase-9.

Western Blot Analysis[4]

Cell Line: NP cells were harvested from the inner tissue of tail disc of SpragueDawley rats
Concentration: 20 μM
Incubation Time: 48 h
Result: Reduced the phosphorylation levels of IKKα/β, IκBα and P65.
Reduced the phosphorylation levels of JNK, ERK and P38.
Reduced the phosphorylation levels of PI3K. AKT and mTOR.
In Vivo

Physalin A (40-80 mg/kg; Intraperitoneal injection; 10 days) has antitumor effect in H292 xenograft tumor model[3].
Physalin A (2 μL 20 μM; Injection of nucleus pulposus of the disc; Single dose) has a protective effect in a rat model of disc degeneration[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male BALB/c mice[3]
Dosage: 40 and 80 mg/kg
Administration: Intraperitoneal injection (i.p.); 10 days
Result: Significantly reduced the mean tumor volume and tumor weight, had the least effect on the weight of mice.
Decreased Tyr705-p-STAT3 levels, and increased caspase-3 activation.
Animal Model: Male Sprague-Dawley rats aged 6-8 weeks old (220±20 g) with intervertebral disc degeneration[4]
Dosage: 2 μL 20 μM
Administration: Injection of nucleus pulposus of the disc; Single dose
Result: Alleviated disc degradation.
Reduced inflammation and fibrosis.
Molecular Weight

526.53

Formula

C28H30O10
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@]([C@@](OC1=O)([H])C2)(OC3=O)[C@@]([C@@]3(CC[C@@]([C@]45C)([H])[C@@]6([H])[C@@H](C=C4CC=CC5=O)O)O)(O[C@@]76O)[C@](C7=O)([H])[C@]2(C1=C)C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation
References
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Physalin A Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Physalin A23027-91-0ApoptosisAutophagyNF-κBp38 MAPKPI3KAktmTORSTATJAKNuclear factor-κBNuclear factor-kappaBPhosphoinositide 3-kinasePKBProtein kinase BMammalian target of RapamycinJanus kinaseWithanolideAnti-inflammatory agent Antifibrotic agentNon-small cell lung cancer cellsQuinone reductaseDisc degenerationInhibitorinhibitorinhibit

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