Procaspase-3/6 activator 1 

Procaspase-3/6 activator 1 (compound 1541) is a highly specific and robust activator of executioner procaspases-3 and -6, with EC₅₀ values of 2.4 ± 0.2 and 2.8 ± 0.3 μM, respectively. Procaspase-3/6 activator 1 does not activate procaspases-1 or -7^[1].

Procaspase-3 at 100 nM was incubated with Procaspase-3/6 activator 1 (compound 1541) (100 nM to 100 μM). After a 4-hour incubation at 37°C, the samples were assayed for caspase activity by addition of Ac-DEVD-AFC. Low concentrations of 1541 induce activity of procaspase-3 with an EC50 of 2.4 μM, and, at high concentrations, inhibit with an IC50 of 34.0 μM^[1].
Procaspase-3/6 activator 1 (compound 1541) exerts a similar inhibitory effect on active caspase-3 (IC50 = 35 μM), as seen for procaspase-3^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

411.41

C₂₄H₁₇N₃O₄

1100353-03-4

Solid

Light yellow to yellow

O=C1C(C(NC2=CC=CC(C3=CN4C=CC=CC4=N3)=C2)=O)=CC5=CC=CC(OC)=C5O1

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Wolan DW, et al. Small-molecule activators of a proenzyme. Science. 2009 Nov 6;326(5954):853-8.  [Content Brief]