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  4. PYCR1-IN-1

PYCR1-IN-1 (compound 4) is a pyrroline-5-carboxylate reductase 1 (PYCR1) inhibitor with an IC50 of 8.8 µM. PYCR1-IN-1 has anticancer effects.

For research use only. We do not sell to patients.

PYCR1-IN-1 Chemical Structure

PYCR1-IN-1 Chemical Structure

CAS No. : 709-85-3

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10 mg USD 50 In-stock
25 mg USD 75 In-stock
50 mg USD 120 In-stock
100 mg USD 195 In-stock
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Based on 1 publication(s) in Google Scholar

PYCR1-IN-1 Related Antibodies

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  • Purity & Documentation

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Description

PYCR1-IN-1 (compound 4) is a pyrroline-5-carboxylate reductase 1 (PYCR1) inhibitor with an IC50 of 8.8 µM. PYCR1-IN-1 has anticancer effects[1].

IC50 & Target

IC50: 8.8 µM (PYCR1)[1]
Molecular Weight

238.12

Formula

C11H12BrN
CAS No.
Appearance

Liquid (Density: 1.330±0.06 g/cm3)

Color

Colorless to light yellow

SMILES

C#CCN(C)CC1=CC=C(Br)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (419.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1996 mL 20.9978 mL 41.9956 mL
5 mM 0.8399 mL 4.1996 mL 8.3991 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (10.50 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.79%

COA (243 KB) HNMR (115 KB) LCMS (133 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.1996 mL 20.9978 mL 41.9956 mL 104.9891 mL
5 mM 0.8399 mL 4.1996 mL 8.3991 mL 20.9978 mL
10 mM 0.4200 mL 2.0998 mL 4.1996 mL 10.4989 mL
15 mM 0.2800 mL 1.3999 mL 2.7997 mL 6.9993 mL
20 mM 0.2100 mL 1.0499 mL 2.0998 mL 5.2495 mL
25 mM 0.1680 mL 0.8399 mL 1.6798 mL 4.1996 mL
30 mM 0.1400 mL 0.6999 mL 1.3999 mL 3.4996 mL
40 mM 0.1050 mL 0.5249 mL 1.0499 mL 2.6247 mL
50 mM 0.0840 mL 0.4200 mL 0.8399 mL 2.0998 mL
60 mM 0.0700 mL 0.3500 mL 0.6999 mL 1.7498 mL
80 mM 0.0525 mL 0.2625 mL 0.5249 mL 1.3124 mL
100 mM 0.0420 mL 0.2100 mL 0.4200 mL 1.0499 mL
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PYCR1-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PYCR1-IN-1709-85-3OthersPYCR1 inhibitorprolineanticancerInhibitorinhibitorinhibit

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