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  4. Pyridaben

Pyridaben is a mitochondrial electron transport inhibitor (METI) acaricide that promotes the formation of damaging oxygen and nitrogen radicals. Pyridaben selectively inhibits complex I (NADH dehydrogenase) with an IC50 value of 2.4 nM (assay sites: rat liver and bovine heart mitochondria). Pyridaben also significantly inhibits rat mitochondrial mtNOS function.

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Pyridaben Chemical Structure

Pyridaben Chemical Structure

CAS No. : 96489-71-3

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Based on 1 publication(s) in Google Scholar

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Pyridaben Related Antibodies

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Description

Pyridaben is a mitochondrial electron transport inhibitor (METI) acaricide that promotes the formation of damaging oxygen and nitrogen radicals. Pyridaben selectively inhibits complex I (NADH dehydrogenase) with an IC50 value of 2.4 nM (assay sites: rat liver and bovine heart mitochondria). Pyridaben also significantly inhibits rat mitochondrial mtNOS function[1][2].

IC50 & Target

Mite

 

In Vitro

Pyridaben (0-10 µM; 3 min) selectively inhibits complex I-III in rat brain submitochondrial particles[1].
Pyridaben (0-10 µM; 3 min) leads to marked decrease in respiratory control (IC50 = 1.7 µM) in a dose-dependent manner, in rat brain mitochondria (malate-glutamate as substrate)[1].
Pyridaben (0-10 µM; 3 min) produces a marked decrease in mtNOS functional activity, when malate-glutamate is used as substrate, but not when the substrate is succinate.[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pyridaben Related Antibodies

Cell Viability Assay[1]

Cell Line: Rat brain submitochondrial particles
Concentration: 0-10 µM
Incubation Time: 3 min
Result: Led to a decrease of NADH-cytochrome reductase activity (complex I-III) and without modification of succinate-cytochrome c reductase activity (complex II-III) and of cytochrome oxidase activity (complex IV)

Cell Viability Assay[1]

Cell Line: Rat brain mitochondria
Concentration: 0-10 µM
Incubation Time: 3 min
Result: Showed a marked and dose-dependent decrease in respiratory control in the presence of malate-glutamate as substrate (IC50=1.7 µM)
Molecular Weight

364.93

Formula

C19H25ClN2OS
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C1C(Cl)=C(SCC2=CC=C(C(C)(C)C)C=C2)C=NN1C(C)(C)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (137.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7403 mL 13.7013 mL 27.4025 mL
5 mM 0.5481 mL 2.7403 mL 5.4805 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.85 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (6.85 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
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% 
+
%
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%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7403 mL 13.7013 mL 27.4025 mL 68.5063 mL
5 mM 0.5481 mL 2.7403 mL 5.4805 mL 13.7013 mL
10 mM 0.2740 mL 1.3701 mL 2.7403 mL 6.8506 mL
15 mM 0.1827 mL 0.9134 mL 1.8268 mL 4.5671 mL
20 mM 0.1370 mL 0.6851 mL 1.3701 mL 3.4253 mL
25 mM 0.1096 mL 0.5481 mL 1.0961 mL 2.7403 mL
30 mM 0.0913 mL 0.4567 mL 0.9134 mL 2.2835 mL
40 mM 0.0685 mL 0.3425 mL 0.6851 mL 1.7127 mL
50 mM 0.0548 mL 0.2740 mL 0.5481 mL 1.3701 mL
60 mM 0.0457 mL 0.2284 mL 0.4567 mL 1.1418 mL
80 mM 0.0343 mL 0.1713 mL 0.3425 mL 0.8563 mL
100 mM 0.0274 mL 0.1370 mL 0.2740 mL 0.6851 mL
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Pyridaben Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pyridaben96489-71-3ParasiteMETIcomplex INADH dehydrogenasemitochondriasubmitochondrial particlesmitesInhibitorinhibitorinhibit

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