2. GPCR/G Protein MAPK/ERK Pathway
  3. Ras Raf
  4. RAS/RAS-RAF-IN-1

RAS/RAS-RAF-IN-1 is a potent RAS and RAS-RAF inhibitor. RAS/RAS-RAF-IN-1 has a KD of 5.0 μΜ-15 μΜ for cyclophilin A (CYPA) binding affinity. RAS/RAS-RAF-IN-1 has antitumor activity.

For research use only. We do not sell to patients.

RAS/RAS-RAF-IN-1 Chemical Structure

RAS/RAS-RAF-IN-1 Chemical Structure

CAS No. : 2447039-81-6

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1 mg USD 480 In-stock
5 mg USD 1400 In-stock
10 mg USD 2250 In-stock
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Based on 1 publication(s) in Google Scholar

RAS/RAS-RAF-IN-1 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE RAS/RAS-RAF-IN-1

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K-Ras H-Ras N-Ras Ras

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BRaf c-Raf Raf

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

RAS/RAS-RAF-IN-1 is a potent RAS and RAS-RAF inhibitor. RAS/RAS-RAF-IN-1 has a KD of 5.0 μΜ-15 μΜ for cyclophilin A (CYPA) binding affinity. RAS/RAS-RAF-IN-1 has antitumor activity[1].

IC50 & Target[1]

Ras

 

RAF

 

In Vitro

RAS/RAS-RAF-IN-1 (compound 89; for 4 hours) has an EC50 of 0.5 μΜ-5.0 μΜ for BRAF-GTP-KRAS-G12C disruption and has an IC50 of 1.0 μΜ-10 μΜ for pERK inhibition in H358 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RAS/RAS-RAF-IN-1 Related Antibodies
Molecular Weight

807.98

Formula

C45H57N7O7
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

CC(C)(C1=CN(CC(C)(COC([C@@](CCC2)([H])NN2C3=O)=O)C)C4=CC(C5=CC(O)=CC(C[C@@H]3NC([C@H](C(C)C)N(C)C([C@@H](CC6)CN6C(C=C)=O)=O)=O)=C5)=CC=C41)C#N
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 200 mg/mL (247.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2377 mL 6.1883 mL 12.3765 mL
5 mM 0.2475 mL 1.2377 mL 2.4753 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 6 mg/mL (7.43 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 6 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (60.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 5 mg/mL (6.19 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2377 mL 6.1883 mL 12.3765 mL 30.9414 mL
5 mM 0.2475 mL 1.2377 mL 2.4753 mL 6.1883 mL
10 mM 0.1238 mL 0.6188 mL 1.2377 mL 3.0941 mL
15 mM 0.0825 mL 0.4126 mL 0.8251 mL 2.0628 mL
20 mM 0.0619 mL 0.3094 mL 0.6188 mL 1.5471 mL
25 mM 0.0495 mL 0.2475 mL 0.4951 mL 1.2377 mL
30 mM 0.0413 mL 0.2063 mL 0.4126 mL 1.0314 mL
40 mM 0.0309 mL 0.1547 mL 0.3094 mL 0.7735 mL
50 mM 0.0248 mL 0.1238 mL 0.2475 mL 0.6188 mL
60 mM 0.0206 mL 0.1031 mL 0.2063 mL 0.5157 mL
80 mM 0.0155 mL 0.0774 mL 0.1547 mL 0.3868 mL
100 mM 0.0124 mL 0.0619 mL 0.1238 mL 0.3094 mL
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RAS/RAS-RAF-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RAS/RAS-RAF-IN-12447039-81-6RasRafRaf kinasesBRAF-GTP-KRAS-G12CdisruptionpERKH358cyclophilin ACYPAInhibitorinhibitorinhibit

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