ADK/Adenosine Kinase Protein, Human (HEK293)

ADK is an enzyme that catalyzes the transfer of gamma-phosphate from adenosine triphosphate (ATP) to adenosine (Ado) to form adenosine monophosphate (AMP). ADK acts as a potential regulator of extracellular adenosine and intracellular adenine nucleotide concentrations. ADK is a therapeutic target for controlling obesity and non-alcoholic fatty liver disease. ADK/Adenosine Kinase Protein, Human (HEK293) is the recombinant human-derived ADK/Adenosine Kinase protein, expressed by HEK293 , with tag free. The total length of ADK/Adenosine Kinase Protein, Human (HEK293) is 345 a.a., with molecular weight of ~38.74 kDa.

Adenosine kinase is an enzyme that catalyzes the transfer of γ-phosphate from adenosine triphosphate (ATP) to adenosine (Ado) to form adenosine monophosphate (AMP). Catalyze the phosphorylation of purine nucleoside adenosine at the 5 'position in an ATP-dependent manner. ADK acts as a potential regulator of extracellular adenosine and intracellular adenine nucleotide concentrations. ADK promotes excess fat deposition and liver inflammation by inhibiting fatty acid oxidation in hepatocytes and producing hepatocellular proinflammatory mediators, which is a therapeutic target for controlling obesity and non-alcoholic fatty liver disease^[1][2][3].

Measured by its ability to phosphorylate Adenosine that incubate at room temperature for 60 minutes. The specific activity is 32.58 pmo/min/μg.

Human

HEK293

Tag Free

AAH03568 (M1-H345)

132  [NCBI]

Approximately 38.74 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 300 mM NaCl pH 8.0, 0.5 mM PMSF, 0.5 mM TCEP, 20% glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.