APLP-1 Protein, Mouse (HEK293, His)

APLP-1 protein is involved in postsynaptic function and synaptic processes. Its processing produces ALID1, which activates transcription through APBB1 binding. APLP-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived APLP-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of APLP-1 Protein, Mouse (HEK293, His) is 584 a.a., with molecular weight of 70-95 kDa.

The APLP-1 protein may have a role in postsynaptic function, suggesting its involvement in the intricate processes occurring at the synaptic sites. The C-terminal fragment resulting from gamma-secretase processing, ALID1, exhibits the ability to activate transcription through binding to APBB1 (Fe65). APLP-1 is also implicated in JIP signal transduction by interacting with the C-terminal region. Moreover, it appears to engage with cellular G-protein signaling pathways, indicating its potential influence on diverse cellular signaling mechanisms. Additionally, APLP-1 can regulate neurite outgrowth by binding to extracellular matrix components such as heparin and collagen I. However, the gamma-CTF peptide, C30, derived from APLP-1, is noteworthy for its potent enhancement of neuronal apoptosis, underscoring the dual nature of APLP-1's functional effects in cellular processes. Further exploration into the specific mechanisms underlying these diverse roles could provide valuable insights into the multifaceted functions of APLP-1 in neural development and homeostasis.

Mouse

HEK293

C-10*His

Q03157 (N39-E584)

11803  [NCBI]

NLAVGSPSAAEAPGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLLDPQRVLEYCRQMYPELHIARVEQAAQAIPMERWCGGTRSGRCAHPHHEVVPFHCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPATPNPSGMAAGDPSTRSWPLGGRAEGGEDEEEVESFPQPVDDYFVEPPQAEEEEEEEEERAPPPSSHTPVMVSRVTPTPRPTDGVDVYFGMPGEIGEHEGFLRAKMDLEERRMRQINEVMREWAMADSQSKNLPKADRQALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQGDPPQAERVLMALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVQTHLQVIEERMNQSLGLLDQNPHLAQELRPQIQELLLAEHLGPSELDASVPGSSSEDKGSLQPPESKDDPPVTLPKGSTDQESSSSGREKLTPLEQYEQKVNASAPRGFPFHSSDIQRDELAPSGTGVSREG

Approximately 70-95 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.