1. Recombinant Proteins
  2. Others
  3. ATG9B Protein, Human (HEK293, FLAG)

ATG9B Protein, Human (HEK293, FLAG)

Cat. No.: HY-P701358
Handling Instructions

ATG9B Protein, a phospholipid scramblase crucial for autophagy, dynamically cycles between the preautophagosomal structure (PAS) and the cytoplasmic vesicle pool. It plays a critical role in expanding autophagosomal membranes, facilitating lipid distribution with ATG2 and driving membrane expansion. Additionally, ATG9B participates in necrotic cell death. ATG9B Protein, Human (HEK293, FLAG) is the recombinant human-derived ATG9B protein, expressed by HEK293 , with C-Flag labeled tag. The total length of ATG9B Protein, Human (HEK293, FLAG) is 924 a.a., .

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Stock
50 μg   Get quote  
100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

ATG9B Protein, a phospholipid scramblase crucial for autophagy, dynamically cycles between the preautophagosomal structure (PAS) and the cytoplasmic vesicle pool. It plays a critical role in expanding autophagosomal membranes, facilitating lipid distribution with ATG2 and driving membrane expansion. Additionally, ATG9B participates in necrotic cell death. ATG9B Protein, Human (HEK293, FLAG) is the recombinant human-derived ATG9B protein, expressed by HEK293 , with C-Flag labeled tag. The total length of ATG9B Protein, Human (HEK293, FLAG) is 924 a.a., .

Background

ATG9B Protein functions as a phospholipid scramblase crucially involved in autophagy, mediating the expansion of autophagosomal membranes. It dynamically cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool, acting as a critical supplier of membranes for the growing autophagosome. The lipid scramblase activity of ATG9B is pivotal in the preautophagosomal structure/phagophore assembly process, facilitating the distribution of phospholipids arriving through ATG2 (either ATG2A or ATG2B) from the cytoplasmic leaflet to the luminal leaflet of the bilayer. This activity, in turn, drives the expansion of autophagosomal membranes. Beyond its role in autophagy, ATG9B also participates in necrotic cell death.

Species

Human

Source

HEK293

Tag

C-Flag

Accession

Q674R7 (M1-D924)

Gene ID

285973

Molecular Construction
N-term
ATG9B (M1-D924)
Accession # Q674R7
Flag
C-term
Synonyms
ATG9B; Autophagy-related protein 9B; APG9-like 2; Nitric oxide synthase 3-overlapping antisense gene protein; Protein sONE
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

ATG9B Protein, Human (HEK293, FLAG) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
ATG9B Protein, Human (HEK293, FLAG)
Cat. No.:
HY-P701358
Quantity:
MCE Japan Authorized Agent: