BAFF/TNFSF13B Protein, Human (HEK293, His-Flag)

BAFF/TNFSF13B protein is a cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA, forming a key ligand-receptor pathway together with TNFSF13/APRIL. These interactions play a crucial role in stimulating B-cell and T-cell function and regulating humoral immunity. BAFF/TNFSF13B Protein, Human (HEK293, His-Flag) is the recombinant human-derived BAFF/TNFSF13B protein, expressed by HEK293 , with N-His, N-Flag labeled tag.

BAFF/TNFSF13B protein, a cytokine, binds to TNFRSF13B/TACI and TNFRSF17/BCMA, forming a key ligand-receptor pathway alongside TNFSF13/APRIL. Together, these interactions play a crucial role in stimulating B- and T-cell function and regulating humoral immunity. Notably, a third B-cell-specific receptor, BAFFR/BR3, is involved in promoting the survival of mature B-cells and facilitating the B-cell response. This intricate network underscores the significance of BAFF/TNFSF13B in orchestrating immune responses. Additionally, isoform 2 of BAFF/TNFSF13B appears to exert a regulatory role by inhibiting the secretion and bioactivity of isoform 1. The dynamic interplay between these isoforms further contributes to the nuanced control of BAFF/TNFSF13B-mediated immune processes.

1. Immobilized Human BAFF Trimer, His Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Human BAFFR, hFc Tag with the EC₅₀ of ≤0.14 μg/mL determined by ELISA.
2. Immobilized Human BAFF Trimer, His Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Human TACI, hFc Tag with the EC₅₀ of ≤8 ng/mL determined by ELISA.
3. Immobilized Human BAFF Trimer, His Tag at 2 μg/mL (100 μl/well) on the plate. Dose response curve for Human BAFFR, hFc Tag with the EC₅₀ of ≤ 60 ng/mL determined by ELISA.

Human

HEK293

N-His;N-Flag

Q9Y275-1 (T141-L285)

10673  [NCBI]

53-60 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.