Nectin-3/CD113 Protein, Human (309a.a, HEK293, His)

CD113 Protein, Human (HEK293, His) is a recombinant human CD113 expressed in HEK 293 cells with a His tag at the N-terminus. CD113 Protein is a member of immunoglobulin-like cell adhesion molecules that shows homophilic and heterophilic cell-cell adhesion activities^[1][2].

Nectin is a Ca²⁺-independent immunoglobulin-like intercellular adhesion molecule. Nectin comprises a family of at least four members nectin-1, nectin-2, nectin-3 and nectin-4. All the members have two or three splice variants (except nectin-4), and have an extracellular region containing three Ig-like domains, a single transmembrane region and a cytoplasmic region (except nectin-1γ). Nectin-1, nectin-2 and nectin-3 are ubiquitously expressed in a variety of cells. Nectin-2 and nectin-3 are also expressed in cells that lack cadherins. Nectin-4 is expressed mainly in the placenta. Nectin-3 has three splicing variants, nectin-3α (biggest), -3β (middle), and -3γ (smallest). Nectin-3α consists of three extracellular immunoglobulin-like domains, a transmembrane segment, and a cytoplasmic region with the C-terminal consensus motif for binding to the PDZ domain^[1][2].

Human

HEK293

C-6*His

Q9NQS3-1 (G58-P366)

25945  [NCBI]

GPIIVEPHVTAVWGKNVSLKCLIEVNETITQISWEKIHGKSSQTVAVHHPQYGFSVQGEYQGRVLFKNYSLNDATITLHNIGFSDSGKYICKAVTFPLGNAQSSTTVTVLVEPTVSLIKGPDSLIDGGNETVAAICIAATGKPVAHIDWEGDLGEMESTTTSFPNETATIISQYKLFPTRFARGRRITCVVKHPALEKDIRYSFILDIQYAPEVSVTGYDGNWFVGRKGVNLKCNADANPPPFKSVWSRLDGQWPDGLLASDNTLHFVHPLTFNYSGVYICKVTNSLGQRSDQKVIYISDPPTTTTLQPHHHHHH

Approximately 54 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, 5% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Takai Y, et, al. Nectin and afadin: novel organizers of intercellular junctions. J Cell Sci. 2003 Jan 1;116(Pt 1):17-27.  [Content Brief]

  [2]. Satoh-Horikawa K, et, al. Nectin-3, a new member of immunoglobulin-like cell adhesion molecules that shows homophilic and heterophilic cell-cell adhesion activities. J Biol Chem. 2000 Apr 7;275(14):10291-9.  [Content Brief]