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  5. Nectin-3/CD113 Protein, Human (309a.a, HEK293, His)

Nectin-3/CD113 Protein, Human (309a.a, HEK293, His)

Cat. No.: HY-P7782
Data Sheet Handling Instructions Technical Support

CD113 Protein, Human (HEK293, His) is a recombinant human CD113 expressed in HEK 293 cells with a His tag at the N-terminus. CD113 Protein is a member of immunoglobulin-like cell adhesion molecules that shows homophilic and heterophilic cell-cell adhesion activities.

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10 μg USD 95 In-stock
50 μg USD 285 In-stock
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Description

CD113 Protein, Human (HEK293, His) is a recombinant human CD113 expressed in HEK 293 cells with a His tag at the N-terminus. CD113 Protein is a member of immunoglobulin-like cell adhesion molecules that shows homophilic and heterophilic cell-cell adhesion activities[1][2].

Background

Nectin is a Ca2+-independent immunoglobulin-like intercellular adhesion molecule. Nectin comprises a family of at least four members nectin-1, nectin-2, nectin-3 and nectin-4. All the members have two or three splice variants (except nectin-4), and have an extracellular region containing three Ig-like domains, a single transmembrane region and a cytoplasmic region (except nectin-1γ). Nectin-1, nectin-2 and nectin-3 are ubiquitously expressed in a variety of cells. Nectin-2 and nectin-3 are also expressed in cells that lack cadherins. Nectin-4 is expressed mainly in the placenta. Nectin-3 has three splicing variants, nectin-3α (biggest), -3β (middle), and -3γ (smallest). Nectin-3α consists of three extracellular immunoglobulin-like domains, a transmembrane segment, and a cytoplasmic region with the C-terminal consensus motif for binding to the PDZ domain[1][2].

Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q9NQS3-1 (G58-P366)

Gene ID
Molecular Construction
N-term
Nectin-3/CD113 (G58-P366)
Accession # Q9NQS3-1
6*His
C-term
Synonyms
rHuCD113, His; Poliovirus Receptor-Related Protein 3; CDw113; Nectin-3; CD113; PVRL3; PRR3
AA Sequence

GPIIVEPHVTAVWGKNVSLKCLIEVNETITQISWEKIHGKSSQTVAVHHPQYGFSVQGEYQGRVLFKNYSLNDATITLHNIGFSDSGKYICKAVTFPLGNAQSSTTVTVLVEPTVSLIKGPDSLIDGGNETVAAICIAATGKPVAHIDWEGDLGEMESTTTSFPNETATIISQYKLFPTRFARGRRITCVVKHPALEKDIRYSFILDIQYAPEVSVTGYDGNWFVGRKGVNLKCNADANPPPFKSVWSRLDGQWPDGLLASDNTLHFVHPLTFNYSGVYICKVTNSLGQRSDQKVIYISDPPTTTTLQPHHHHHH

Molecular Weight

Approximately 54 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, 5% Trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

Nectin-3/CD113 Protein, Human (309a.a, HEK293, His) Related Classifications

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Nectin-3/CD113 Protein, Human (309a.a, HEK293, His)
Cat. No.:
HY-P7782
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