CD23/Fc epsilon RII Protein, Mouse (HEK293, His)

CD23/Fc epsilon RII Protein, a low-affinity receptor for IgE and CR2/CD21, regulates IgE production and B cell differentiation. On B cells, it initiates IgE-dependent antigen uptake and presentation to T cells. On macrophages, IgE binding induces intracellular killing of parasites through the L-Arginine-nitric oxide pathway. CD23/Fc epsilon RII homotrimer interacts with IGHE to regulate IgE homeostasis and with CR2/CD21 for binding. CD23/Fc epsilon RII Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD23/Fc epsilon RII protein, expressed by HEK293 , with N-His labeled tag. The total length of CD23/Fc epsilon RII Protein, Mouse (HEK293, His) is 282 a.a., with molecular weight of 33-43 kDa.

The CD23/Fc epsilon RII protein serves as a low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21, playing crucial roles in the regulation of IgE production and B cell differentiation. On B cells, it initiates IgE-dependent antigen uptake and presentation to T cells, contributing to immune response modulation. In macrophages, CD23, upon binding IgE and cross-linking with antigens, triggers intracellular killing of parasites through the activation of the L-Arginine-nitric oxide pathway. Existing as a homotrimer, CD23 interacts with IGHE, regulating IgE homeostasis through its C-type lectin domain. Additionally, CD23 interacts with CR2/CD21 through its C-terminus, forming a connection via the Sushi domains 1 and 2. These interactions underscore the multifaceted involvement of CD23 in immunological processes, influencing both B cell function and macrophage-mediated responses.

Immobilized recombinant mouse CD23 at 5 μg/ml (100 μl/well) can bind Biotinylated Recombinant mouse IgE Protein. The ED₅₀ for this effect is 0.1294 μg/mL.

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  Immobilized recombinant mouse CD23 at 5 μg/ml (100 μl/well) can bind Biotinylated Recombinant mouse IgE Protein. The ED₅₀ for this effect is 0.1294 μg/mL.

Mouse

HEK293

N-His

P20693-1 (E50-P331)

14128  [NCBI]

33-43 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.