CD26/Dipeptidyl Peptidase 4 Protein, Human (HEK293, Fc)

The CD26/dipeptidyl peptidase 4 protein is a cell surface glycoprotein receptor that induces T cell proliferation and NF-kappa-B activation. CD26 also works with FAP to promote pericellular proteolysis, endothelial cell migration, and invasion of the extracellular matrix. CD26/Dipeptidyl Peptidase 4 Protein, Human (HEK293, Fc) is the recombinant human-derived CD26/Dipeptidyl Peptidase 4 protein, expressed by HEK293 , with N-hFc labeled tag. The total length of CD26/Dipeptidyl Peptidase 4 Protein, Human (HEK293, Fc) is 738 a.a., with molecular weight of 120-130 kDa.

CD26/Dipeptidyl Peptidase 4 protein, a cell surface glycoprotein receptor, plays a pivotal role in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Functioning as a positive regulator of T-cell coactivation, CD26 interacts with ADA, CAV1, IGF2R, and PTPRC, leading to T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its binding to CAV1 and CARD11 induces T-cell proliferation, while its interaction with ADA regulates lymphocyte-epithelial cell adhesion. In association with FAP, CD26 is involved in pericellular proteolysis of the extracellular matrix, contributing to the migration and invasion of endothelial cells. Additionally, it may play a role in promoting lymphatic endothelial cell adhesion, migration, and tube formation. Acting as a serine exopeptidase with dipeptidyl peptidase activity, CD26 regulates various physiological processes by cleaving peptides in the circulation, including chemokines, mitogenic growth factors, neuropeptides, and peptide hormones. Notably, CD26 acts as a receptor for the human coronavirus MERS-CoV-2 during microbial infection.

Measured by its ability to bind Cynomolgus CXCL12 in a functional ELISA. Loaded CD26/Dipeptidyl Peptidase 4 Protein, Human (HEK293, Fc) on ProA Biosensor, can bind MERS-CoV Spike/RBD Protein fragment (RBD, aa 367-606, His ) with an affinity constant of 1.04 nM.

Human

HEK293

N-hFc

P27487 (N29-P766)

1803  [NCBI]

NKGTDDATADSRKTYTLTDYLKNTYRLKLYSLRWISDHEYLYKQENNILVFNAEYGNSSVFLENSTFDEFGHSINDYSISPDGQFILLEYNYVKQWRHSYTASYDIYDLNKRQLITEERIPNNTQWVTWSPVGHKLAYVWNNDIYVKIEPNLPSYRITWTGKEDIIYNGITDWVYEEEVFSAYSALWWSPNGTFLAYAQFNDTEVPLIEYSFYSDESLQYPKTVRVPYPKAGAVNPTVKFFVVNTDSLSSVTNATSIQITAPASMLIGDHYLCDVTWATQERISLQWLRRIQNYSVMDICDYDESSGRWNCLVARQHIEMSTTGWVGRFRPSEPHFTLDGNSFYKIISNEEGYRHICYFQIDKKDCTFITKGTWEVIGIEALTSDYLYYISNEYKGMPGGRNLYKIQLSDYTKVTCLSCELNPERCQYYSVSFSKEAKYYQLRCSGPGLPLYTLHSSVNDKGLRVLEDNSALDKMLQNVQMPSKKLDFIILNETKFWYQMILPPHFDKSKKYPLLLDVYAGPCSQKADTVFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHAINRRLGTFEVEDQIEAARQFSKMGFVDNKRIAIWGWSYGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYTERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEYLLIHGTADDNVHFQQSAQISKALVDVGVDFQAMWYTDEDHGIASSTAHQHIYTHMSHFIKQCFSLP

120-130 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.