1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Transferases (EC 2) Serine/Threonine Kinase Proteins
  4. Cyclin-Dependent Kinases (CDKs)
  5. CDK8-CCNC-MED12 Protein, Human (Sf9, GST, FLAG, His)

CDK8-CCNC-MED12 Protein, Human (Sf9, GST, FLAG, His)

Cat. No.: HY-P701373
Handling Instructions

The CDK8-CCNC-MED12 protein is an important component of the mediator complex, acting as a bridge for gene transcription regulation, transmitting information from gene-specific regulatory factors to RNA polymerase II. It is recruited to the promoter, assembles a preinitiation complex, and promotes transcription. CDK8-CCNC-MED12 Protein, Human (Sf9, GST, FLAG, His) is the recombinant human-derived CDK8-CCNC-MED12 protein, expressed by Sf9 insect cells , with N-6*His, N-Flag, N-GST labeled tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Stock
50 μg   Get quote  
100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

The CDK8-CCNC-MED12 protein is an important component of the mediator complex, acting as a bridge for gene transcription regulation, transmitting information from gene-specific regulatory factors to RNA polymerase II. It is recruited to the promoter, assembles a preinitiation complex, and promotes transcription. CDK8-CCNC-MED12 Protein, Human (Sf9, GST, FLAG, His) is the recombinant human-derived CDK8-CCNC-MED12 protein, expressed by Sf9 insect cells , with N-6*His, N-Flag, N-GST labeled tag.

Background

The CDK8-CCNC-MED12 protein is a crucial component of the Mediator complex, a coactivator integral to the regulation of gene transcription for nearly all RNA polymerase II-dependent genes. As part of the Mediator complex, it acts as a bridge, conveying information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. This complex is recruited to promoters through direct interactions with regulatory proteins, serving as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and general transcription factors. CDK8-CCNC-MED12 exerts its regulatory functions by phosphorylating the C-terminal domain (CTD) of the large subunit of RNA polymerase II, potentially inhibiting the formation of a transcription initiation complex. Furthermore, it phosphorylates CCNH, leading to the down-regulation of the TFIIH complex and subsequent transcriptional repression. Notably, CDK8-CCNC-MED12 is recruited through interaction with MAML1, contributing to the hyperphosphorylation of the intracellular domain of NOTCH and facilitating its degradation. These multifaceted roles underscore the significance of CDK8-CCNC-MED12 in orchestrating the intricate processes of gene transcription regulation.

Species

Human

Source

Sf9 insect cells

Tag

N-Flag;N-GST;N-His

Accession

P49336-1 (M1-Y464)&P24863-1 (M1-S283)&Q93074-1 (M1-D100)

Gene ID

1024&892&9968

Synonyms
CDK8; CCNC; MED12; Cyclin-dependent kinase 8; Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35; Cyclin-C; SRB11 homolog; hSRB11; Mediator of RNA polymerase II transcription subunit 12; Activator-recruited cofactor 240 kDa component; ARC240; CAG repeat protein 45; Mediator complex subunit 12; OPA-containing protein; Thyroid hormone receptor-associated protein complex 230 kDa component; Trap230; Trinucleotide repeat-containing gene 11 protein
Molecular Weight

79.8 kDa&36 kDa&12.2 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

CDK8-CCNC-MED12 Protein, Human (Sf9, GST, FLAG, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
CDK8-CCNC-MED12 Protein, Human (Sf9, GST, FLAG, His)
Cat. No.:
HY-P701373
Quantity:
MCE Japan Authorized Agent: