Chordin-like 1/CHRDL1 Protein, Human (N-His)

The CHRDL1 protein acts as a BMP4 antagonist, blocking its receptor interaction and redirecting neural stem cells from gliogenesis to neurogenesis. The role of CHRDL1 in preventing glial cell fate adoption supports neuronal differentiation in the brain. Chordin-like 1/CHRDL1 Protein, Human (N-His) is the recombinant human-derived Chordin-like 1/CHRDL1 protein, expressed by E. coli , with N-His labeled tag.

The Chordin-like 1 (CHRDL1) protein serves as an antagonist to BMP4 by binding to it, thus impeding its interaction with receptors. This interaction leads to a shift in the fate commitment of neural stem cells, steering them away from gliogenesis towards neurogenesis. CHRDL1's involvement in preventing the adoption of a glial fate contributes to the neuronal differentiation of neural stem cells in the brain. Beyond its role in neural development, CHRDL1 may play a crucial part in dorsoventral axis formation and embryonic bone development (By similarity). Additionally, CHRDL1 is implicated in regulating retinal angiogenesis by modulating BMP4 actions in endothelial cells. Its participation in anterior segment eye development underscores its multifaceted role in various developmental processes.

Immobilized Human CHRDL1, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CHRDL1 Antibody, hFc Tag with the EC₅₀ of 9.4 ng/ml determined by ELISA.

Human

E. coli

N-His

Q9BU40-1 (E28-C456)

91851

Approximately 50 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 4 mM HCl, pH 4.0. Normally 8 % trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.