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DMP-1 Protein, Human (HEK293, His)

Cat. No.: HY-P70105
COA Handling Instructions

DMP-1 protein exhibits dual functions in osteoblast differentiation. In undifferentiated osteoblast nuclei, its nonphosphorylated form activates osteoblast-specific genes. DMP-1 Protein, Human (HEK293, His) is the recombinant human-derived DMP-1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of DMP-1 Protein, Human (HEK293, His) is 497 a.a., with molecular weight of 53-90 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

DMP-1 protein exhibits dual functions in osteoblast differentiation. In undifferentiated osteoblast nuclei, its nonphosphorylated form activates osteoblast-specific genes. DMP-1 Protein, Human (HEK293, His) is the recombinant human-derived DMP-1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of DMP-1 Protein, Human (HEK293, His) is 497 a.a., with molecular weight of 53-90 kDa.

Background

DMP-1 Protein appears to exhibit a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, its unphosphorylated form functions as a transcriptional component, activating osteoblast-specific genes such as osteocalcin. As osteoblasts transition to osteocytes, DMP-1 undergoes phosphorylation and is subsequently exported into the extracellular matrix. In this extracellular environment, it plays a regulatory role in the nucleation of hydroxyapatite, suggesting its involvement in the mineralization process. DMP-1's intricate functions underscore its significance in the molecular events that govern osteoblast differentiation and bone formation. Notably, DMP-1 interacts with importin alpha, adding a layer of complexity to its cellular dynamics and potential regulatory mechanisms.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized Integrin alpha v beta 3 at 2 µg/mL can bind DMP-1. The ED50 for this effect is 6.245-6.798 nM.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q13316 (L17-Y513)

Gene ID
Molecular Construction
N-term
DMP-1 (L17-Y513)
Accession # Q13316
6*His
C-term
Synonyms
rHuDentin matrix acidic phosphoprotein 1/DMP-1, His; Dentin Matrix Acidic Phosphoprotein 1; DMP-1; Dentin Matrix Protein 1; DMP1
AA Sequence

LPVTRYQNNESEDSEEWKGHLAQAPTPPLESSESSEGSKVSSEEQANEDPSDSTQSEEGLGSDDHQYIYRLAGGFSRSTGKGGDDKDDDEDDSGDDTFGDDDSGPGPKDRQEGGNSRLGSDEDSDDTIQASEESAPQGQDSAQDTTSESRELDNEDRVDSKPEGGDSTQESESEEHWVGGGSDGESSHGDGSELDDEGMQSDDPESIRSERGNSRMNSAGMKSKESGENSEQANTQDSGGSQLLEHPSRKIFRKSRISEEDDRSELDDNNTMEEVKSDSTENSNSRDTGLSQPRRDSKGDSQEDSKENLSQEESQNVDGPSSESSQEANLSSQENSSESQEEVVSESRGDNPDPTTSYVEDQEDSDSSEEDSSHTLSHSKSESREEQADSESSESLNFSEESPESPEDENSSSQEGLQSHSSSAESQSEESHSEEDDSDSQDSSRSKEDSNSTESKSSSEEDGQLKNIEIESRKLTVDAYHNKPIGDQDDNDCQDGY

Molecular Weight

35-95 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Histidine-HCl, 6% Trehalose, 4% Mannitol, 0.05% Tween 80, pH 6.0 or PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

DMP-1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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DMP-1 Protein, Human (HEK293, His)
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