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  3. DMP-1 Protein, Human (HEK293, His)

DMP-1 protein exhibits dual functions in osteoblast differentiation. In undifferentiated osteoblast nuclei, its nonphosphorylated form activates osteoblast-specific genes. DMP-1 Protein, Human (HEK293, His) is the recombinant human-derived DMP-1 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.

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Free Sample   Apply now
5 μg USD 30 In-stock
10 μg USD 45 In-stock
50 μg USD 120 In-stock
100 μg USD 200 In-stock
500 μg USD 500 In-stock
1 mg USD 700 In-stock
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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

DMP-1 protein exhibits dual functions in osteoblast differentiation. In undifferentiated osteoblast nuclei, its nonphosphorylated form activates osteoblast-specific genes. DMP-1 Protein, Human (HEK293, His) is the recombinant human-derived DMP-1 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

DMP-1 Protein appears to exhibit a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, its unphosphorylated form functions as a transcriptional component, activating osteoblast-specific genes such as osteocalcin. As osteoblasts transition to osteocytes, DMP-1 undergoes phosphorylation and is subsequently exported into the extracellular matrix. In this extracellular environment, it plays a regulatory role in the nucleation of hydroxyapatite, suggesting its involvement in the mineralization process. DMP-1's intricate functions underscore its significance in the molecular events that govern osteoblast differentiation and bone formation. Notably, DMP-1 interacts with importin alpha, adding a layer of complexity to its cellular dynamics and potential regulatory mechanisms.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized Integrin alpha v beta 3 at 2 µg/mL can bind DMP-1. The ED50 for this effect is 6.245-6.798 nM.

  • Measured by its binding ability in a functional ELISA. Immobilized Integrin alpha v beta 3 at 2 µg/mL can bind DMP-1 .The ED50 for this effect is 6.245 nM.
Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q13316 (L17-Y513)

Gene ID
Molecular Construction
N-term
DMP-1 (L17-Y513)
Accession # Q13316
6*His
C-term
Synonyms
rHuDentin matrix acidic phosphoprotein 1/DMP-1, His; Dentin Matrix Acidic Phosphoprotein 1; DMP-1; Dentin Matrix Protein 1; DMP1
AA Sequence

LPVTRYQNNESEDSEEWKGHLAQAPTPPLESSESSEGSKVSSEEQANEDPSDSTQSEEGLGSDDHQYIYRLAGGFSRSTGKGGDDKDDDEDDSGDDTFGDDDSGPGPKDRQEGGNSRLGSDEDSDDTIQASEESAPQGQDSAQDTTSESRELDNEDRVDSKPEGGDSTQESESEEHWVGGGSDGESSHGDGSELDDEGMQSDDPESIRSERGNSRMNSAGMKSKESGENSEQANTQDSGGSQLLEHPSRKIFRKSRISEEDDRSELDDNNTMEEVKSDSTENSNSRDTGLSQPRRDSKGDSQEDSKENLSQEESQNVDGPSSESSQEANLSSQENSSESQEEVVSESRGDNPDPTTSYVEDQEDSDSSEEDSSHTLSHSKSESREEQADSESSESLNFSEESPESPEDENSSSQEGLQSHSSSAESQSEESHSEEDDSDSQDSSRSKEDSNSTESKSSSEEDGQLKNIEIESRKLTVDAYHNKPIGDQDDNDCQDGY

Molecular Weight

35-95 kDa due to glycosylation.

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Histidine-HCl, 6% Trehalose, 4% Mannitol, 0.05% Tween 80, pH 6.0 or PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

DMP-1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Inquiry Information

Product Name:
DMP-1 Protein, Human (HEK293, His)
Cat. No.:
HY-P70105
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