DMP-1 Protein, Human (HEK293, His)

DMP-1 protein exhibits dual functions in osteoblast differentiation. In undifferentiated osteoblast nuclei, its nonphosphorylated form activates osteoblast-specific genes. DMP-1 Protein, Human (HEK293, His) is the recombinant human-derived DMP-1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of DMP-1 Protein, Human (HEK293, His) is 497 a.a., with molecular weight of 53-90 kDa.

DMP-1 Protein appears to exhibit a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, its unphosphorylated form functions as a transcriptional component, activating osteoblast-specific genes such as osteocalcin. As osteoblasts transition to osteocytes, DMP-1 undergoes phosphorylation and is subsequently exported into the extracellular matrix. In this extracellular environment, it plays a regulatory role in the nucleation of hydroxyapatite, suggesting its involvement in the mineralization process. DMP-1's intricate functions underscore its significance in the molecular events that govern osteoblast differentiation and bone formation. Notably, DMP-1 interacts with importin alpha, adding a layer of complexity to its cellular dynamics and potential regulatory mechanisms.

Measured by its binding ability in a functional ELISA. Immobilized Integrin alpha v beta 3 at 2 µg/mL can bind DMP-1. The ED₅₀ for this effect is 6.245-6.798 nM.

Human

HEK293

C-6*His

Q13316 (L17-Y513)

1758  [NCBI]

LPVTRYQNNESEDSEEWKGHLAQAPTPPLESSESSEGSKVSSEEQANEDPSDSTQSEEGLGSDDHQYIYRLAGGFSRSTGKGGDDKDDDEDDSGDDTFGDDDSGPGPKDRQEGGNSRLGSDEDSDDTIQASEESAPQGQDSAQDTTSESRELDNEDRVDSKPEGGDSTQESESEEHWVGGGSDGESSHGDGSELDDEGMQSDDPESIRSERGNSRMNSAGMKSKESGENSEQANTQDSGGSQLLEHPSRKIFRKSRISEEDDRSELDDNNTMEEVKSDSTENSNSRDTGLSQPRRDSKGDSQEDSKENLSQEESQNVDGPSSESSQEANLSSQENSSESQEEVVSESRGDNPDPTTSYVEDQEDSDSSEEDSSHTLSHSKSESREEQADSESSESLNFSEESPESPEDENSSSQEGLQSHSSSAESQSEESHSEEDDSDSQDSSRSKEDSNSTESKSSSEEDGQLKNIEIESRKLTVDAYHNKPIGDQDDNDCQDGY

35-95 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Histidine-HCl, 6% Trehalose, 4% Mannitol, 0.05% Tween 80, pH 6.0 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.