EBP50/SLC9A3R1 Protein, Human (HEK293, His)

EBP50 is a scaffolding protein that connects plasma membrane proteins to the actin cytoskeleton, regulates its surface expression and forms homo- or heterodimers with NHERF2. It regulates the activity and localization of SLC9A3 and is critical for the recycling of ADRB2. EBP50/SLC9A3R1 Protein, Human (HEK293, His) is the recombinant human-derived EBP50/SLC9A3R1, expressed by HEK293 , with C-6*His labeled tag.

EBP50 serves as a scaffold protein, facilitating the connection between plasma membrane proteins and members of the ezrin/moesin/radixin family, thereby linking them to the actin cytoskeleton and regulating their surface expression. Essential for the recycling of internalized ADRB2, EBP50 plays a crucial role in the regulation of SLC9A3 activity and subcellular localization. It is indispensable for cAMP-mediated phosphorylation and inhibition of SLC9A3 and may contribute to Wnt signaling and the targeting of HTR4 to microvilli. Furthermore, EBP50 is involved in phosphate reabsorption in renal proximal tubules, sperm capacitation, and the regulation of chloride and bicarbonate homeostasis in spermatozoa. It forms homodimers and heterodimers with NHERF2 and interacts with a diverse array of proteins, including ezrin, radixin, moesin, PDGFRA, PDGFRB, ADRB2, NOS2, CFTR, ARHGAP17, EPI64, RACK1, OPRK1, GNAQ, CTNNB1, PLCB3, and PDZK1. Additionally, EBP50 participates in complexes with CLCN3, PAG1, CFTR, SLC4A7, ATP6V1B1, TRPC4, HTR4, PODXL, SLC26A3, MCC, SLC34A1, SLC26A6, and ACE2, highlighting its diverse roles in cellular processes and signaling pathways.

Human

HEK293

C-6*His

O14745-1 (M1-L358)

9368

MSADAAAGAPLPRLCCLEKGPNGYGFHLHGEKGKLGQYIRLVEPGSPAEKAGLLAGDRLVEVNGENVEKETHQQVVSRIRAALNAVRLLVVDPETDEQLQKLGVQVREELLRAQEAPGQAEPPAAAEVQGAGNENEPREADKSHPEQRELRPRLCTMKKGPSGYGFNLHSDKSKPGQFIRSVDPDSPAEASGLRAQDRIVEVNGVCMEGKQHGDVVSAIRAGGDETKLLVVDRETDEFFKKCRVIPSQEHLNGPLPVPFTNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL

Approximately 48-60 kDa due to the phosphorylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.