ENTE Protein, S. aureus (N-His)

ENTE Proteinas are staphylococcal enterotoxins that activate the host immune system by binding to major histocompatibility complex class II and T-cell receptor (TCR) molecules. This interaction forms a ternary complex that activates T lymphocytes and induces the systemic release of proinflammatory cytokines. ENTE Protein, S. aureus (N-His) is the recombinant Staphylococcus aureus-derived ENTE protein, expressed by E. coli , with N-6*His labeled tag. The total length of ENTE Protein, S. aureus (N-His) is 230 a.a., with molecular weight of ~27 kDa.

ENTE, a staphylococcal enterotoxin, plays a pivotal role in activating the host immune system by binding as unprocessed molecules to major histocompatibility complex class II and T-cell receptor (TCR) molecules. This interaction results in the formation of a ternary complex, which, in turn, activates a significant number of T-lymphocytes, triggering a systemic release of pro-inflammatory cytokines. Beyond its immunomodulatory functions, ENTE is implicated in the development of staphylococcal food poisoning syndrome, a condition characterized by symptoms such as intoxication, fever, and gastrointestinal distress. These activities highlight the impact of ENTE on both immune response pathways and pathological outcomes.

Staphylococcus aureus

E. coli

N-6*His

P12993 (S28-T257)

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SEEINEKDLRKKSELQRNALSNLRQIYYYNEKAITENKESDDQFLENTLLFKGFFTGHPWYNDLLVDLGSKDATNKYKGKKVDLYGAYYGYQCAGGTPNKTACMYGGVTLHDNNRLTEEKKVPINLWIDGKQTTVPIDKVKTSKKEVTVQELDLQARHYLHGKFGLYNSDSFGGKVQRGLIVFHSSEGSTVSYDLFDAQGQYPDTLLRIYRDNKTINSENLHIDLYLYTT

Approximately 27 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For lower concentration, please reconstitute in 50 mM Tris-HCL,300 mM NaCl, pH 7.4 buffer.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.