EphA2 Protein, Human (Biotinylated, HEK293, His-Avi)

The EphA2 protein is an important member of the ephrin receptor subfamily that mediates developmental events, particularly in the nervous system. It has a single kinase domain and an extracellular region that binds ephrin-A ligands. EphA2 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived EphA2 protein, expressed by HEK293 , with C-6*His, C-Avi labeled tag. The total length of EphA2 Protein, Human (Biotinylated, HEK293, His-Avi) is 511 a.a., with molecular weight of ~59.4 kDa.

EphA2 Protein, a member of the ephrin receptor subfamily within the protein-tyrosine kinase family, plays a crucial role in mediating developmental events, particularly within the nervous system. Featuring a single kinase domain and an extracellular region with a Cys-rich domain and 2 fibronectin type III repeats, EPH receptors are involved in binding ephrin-A and ephrin-B ligands. EphA2 specifically binds ephrin-A ligands and displays broad expression across various tissues, including esophagus (RPKM 37.8), urinary bladder (RPKM 18.3), and 21 other tissues. Additionally, mutations in this gene are associated with certain genetically-related cataract disorders, highlighting its significance in both developmental processes and ocular health.

Measured by its binding ability in a functional ELISA. Immobilized EFNA1-hFc at 10 μg/mL (100 μL/well) can bind Biotinylated EphA2 Protein, Human (HEK293, His-Avi) and the EC₅₀ is 20-40 ng/mL.

Human

HEK293

C-6*His;C-Avi

NP_004422.2 (A24-N534)

1969  [NCBI]

LKTYVDPHTYEDPNQAVLKFTTEIHPSCVTRQKVIGAGEFGEVYKGMLKTSSGKKEVPVAIKTLKAGYTEKQRVDFLGEAGIMGQFSHHNIIRLEGVISKYKPMMIITEYMENGALDKFLREKDGEFSVLQLVGMLRGIAAGMKYLANMNYVHRDLAARNILVNSNLVCKVSDFGLSRVLEDDPEATYTTSGGKIPIRWTAPEAISYRKFTSASDVWSFGIVMWEVMTYGERPYWELSNHEVMKAINDGFRLPTPMDCPSAIYQLMMQCWQQERARRPKFADIVSILDKLIRAPDSLKTLADFDPRVSIRLPSTSGSEGVPFRTVSEWLESIKMQQYTEHFMAAGYTAIEKVVQMTNDDIKRIGVRLPGHQKRIAYSLLGLKDQVNTVGIPI

Approximately 59.4 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.