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  3. Follistatin-like 1/FSTL1 Protein, Human (HEK293, His)

Follistatin-like 1/FSTL1 Protein, Human (HEK293, His)

Cat. No.: HY-P70412
COA Handling Instructions

Follistatin-like 1 (FSTL1) protein is a secreted glycoprotein that contributes to angiogenesis, immune response regulation, and cell proliferation. It aids in the development of the central nervous system, skeletal system, lungs and ureters. Follistatin-like 1/FSTL1 Protein, Human (HEK293, His) is the recombinant human-derived Follistatin-like 1/FSTL1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Follistatin-like 1/FSTL1 Protein, Human (HEK293, His) is 288 a.a., with molecular weight of ~48.0 kDa.

For research use only. We do not sell to patients.

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50 μg $280 In-stock
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  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

Follistatin-like 1 (FSTL1) protein is a secreted glycoprotein that contributes to angiogenesis, immune response regulation, and cell proliferation. It aids in the development of the central nervous system, skeletal system, lungs and ureters. Follistatin-like 1/FSTL1 Protein, Human (HEK293, His) is the recombinant human-derived Follistatin-like 1/FSTL1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Follistatin-like 1/FSTL1 Protein, Human (HEK293, His) is 288 a.a., with molecular weight of ~48.0 kDa.

Background

Follistatin-like 1 (FSTL1) protein is a secreted glycoprotein that plays a role in various physiological processes, including angiogenesis, immune response regulation, cell proliferation, and differentiation. It is involved in the development of the central nervous system, skeletal system, lungs, and ureter. FSTL1 promotes endothelial cell survival, migration, and differentiation into network structures through an AKT-dependent mechanism. It also supports the survival of cardiac myocytes. FSTL1 initiates different signaling cascades by activating various receptors on the cell surface, such as DIP2A, TLR4, or BMP receptors. It forms homodimers and interacts with SCN10A. FSTL1 also interacts with DIP2A, which may act as a cell surface receptor for FSTL1. Additionally, FSTL1 interacts with BMP4 and CD14, with the latter interaction promoting TLR4-mediated signaling cascade.

Biological Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Follistatin‑like 1/FSTL1 is coated at 2 μg/mL (100 μL/well) can bind Recombinant human BMP-4. The ED50 for this effect is 40.46 ng/mL.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q12841 (E21-I308)

Gene ID
Molecular Construction
N-term
FSTL1 (E21-I308)
Accession # Q12841
6*His
C-term
Synonyms
rHuFollistatin-related protein 1/FSTL1, His; Follistatin-Related Protein 1; Follistatin-Like Protein 1; FSTL1; FRP
AA Sequence

EEELRSKSKICANVFCGAGRECAVTEKGEPTCLCIEQCKPHKRPVCGSNGKTYLNHCELHRDACLTGSKIQVDYDGHCKEKKSVSPSASPVVCYQSNRDELRRRIIQWLEAEIIPDGWFSKGSNYSEILDKYFKNFDNGDSRLDSSEFLKFVEQNETAINITTYPDQENNKLLRGLCVDALIELSDENADWKLSFQEFLKCLNPSFNPPEKKCALEDETYADGAETEVDCNRCVCACGNWVCTAMTCDGKNQKGAQTQTEEEMTRYVQELQKHQETAEKTKRVSTKEI

Molecular Weight

Approximately 48.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

Follistatin-like 1/FSTL1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Follistatin-like 1/FSTL1 Protein, Human (HEK293, His)
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HY-P70412
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