FUT8 Protein, Hamster (sf9, His)

FUT8 protein catalyzes the addition of fucose to the initial GlcNAc residue in N-glycans. FUT8 Protein, Hamster (sf9, His) is the recombinant FUT8 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of FUT8 Protein, Hamster (sf9, His) is 508 a.a., with molecular weight of ~55 kDa.

FUT8 protein acts as an enzyme responsible for catalyzing the addition of fucose in an alpha 1-6 linkage to the initial GlcNAc residue that is adjacent to peptide chains in N-glycans.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Others

Sf9 insect cells

C-His

G3HCE4 (R68-K575)

100751648  [NCBI]

Approximately 55 kDa

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 3 mM DTT, 10% Glycerol, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.