1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. FUT8 Protein, Hamster (sf9, His)

FUT8 Protein, Hamster (sf9, His)

Cat. No.: HY-P75783
COA Handling Instructions

FUT8 protein catalyzes the addition of fucose to the initial GlcNAc residue in N-glycans. FUT8 Protein, Hamster (sf9, His) is the recombinant FUT8 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of FUT8 Protein, Hamster (sf9, His) is 508 a.a., with molecular weight of ~55 kDa.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
50 μg $850 In-stock
100 μg   Get quote  

* Please select Quantity before adding items.

FUT8 Protein, Hamster (sf9, His) Featured Recommendations:

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

FUT8 protein catalyzes the addition of fucose to the initial GlcNAc residue in N-glycans. FUT8 Protein, Hamster (sf9, His) is the recombinant FUT8 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of FUT8 Protein, Hamster (sf9, His) is 508 a.a., with molecular weight of ~55 kDa.

Background

FUT8 protein acts as an enzyme responsible for catalyzing the addition of fucose in an alpha 1-6 linkage to the initial GlcNAc residue that is adjacent to peptide chains in N-glycans.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Others

Source

Sf9 insect cells

Tag

C-His

Accession

G3HCE4 (R68-K575)

Gene ID

100751648  [NCBI]

Molecular Construction
N-term
FUT8 (R68-K575)
Accession # G3HCE4
His
C-term
Synonyms
Alpha-(1,6)-fucosyltransferase; Alpha1-6FucT; FUT8; Fucosyltransferase 8
Molecular Weight

Approximately 55 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 3 mM DTT, 10% Glycerol, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

FUT8 Protein, Hamster (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
FUT8 Protein, Hamster (sf9, His)
Cat. No.:
HY-P75783
Quantity:
MCE Japan Authorized Agent: