Gastric lipase Protein, Human (HEK293, C-His)

Gastric lipase is a key enzyme in the digestive process and plays a key role in the hydrolysis of triacylglycerols into free fatty acids, diacylglycerols, monoacylglycerols, and glycerol. This enzyme activity contributes significantly to the breakdown of dietary fat in the gastric environment. Gastric lipase Protein, Human (HEK293, C-His) is the recombinant human-derived Gastric lipase protein, expressed by HEK293 , with C-6*His labeled tag.

Gastric lipase is an enzyme that catalyzes the hydrolysis of triacylglycerols, breaking them down into free fatty acids, diacylglycerol, monoacylglycerol, and glycerol. This hydrolytic activity plays a crucial role in the digestion of dietary fats in the stomach. Gastric lipase exhibits a preference for hydrolyzing the sn-3 position of triacylglycerol molecules. By selectively targeting specific bonds within triacylglycerols, gastric lipase contributes to the generation of smaller lipid molecules that can be further processed and absorbed during the digestive process. It has to emphasize the enzyme's substrate specificity and its significance in the initial steps of fat digestion.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

P07098-1 (L20-K398)

8513  [NCBI]

LFGKLHPGSPEVTMNISQMITYWGYPNEEYEVVTEDGYILEVNRIPYGKKNSGNTGQRPVVFLQHGLLASATNWISNLPNNSLAFILADAGYDVWLGNSRGNTWARRNLYYSPDSVEFWAFSFDEMAKYDLPATIDFIVKKTGQKQLHYVGHSQGTTIGFIAFSTNPSLAKRIKTFYALAPVATVKYTKSLINKLRFVPQSLFKFIFGDKIFYPHNFFDQFLATEVCSREMLNLLCSNALFIICGFDSKNFNTSRLDVYLSHNPAGTSVQNMFHWTQAVKSGKFQAYDWGSPVQNRMHYDQSQPPYYNVTAMNVPIAVWNGGKDLLADPQDVGLLLPKLPNLIYHKEIPFYNHLDFIWAMDAPQEVYNDIVSMISEDKK

Predict MW: 44kDa; Approximately 48-50 kDa due to glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.