GBA/Glucosylceramidase Protein, Mouse (HEK293, His)

GBA/glucosylceramidase protein catalyzes the hydrolysis of glucosylceramide/GlcCers into free ceramide and glucose within lysosomes. It plays a key role in lipid degradation and cell membrane turnover. GBA/Glucosylceramidase Protein, Mouse (HEK293, His) is the recombinant mouse-derived GBA/Glucosylceramidase protein, expressed by HEK293 , with N-10*His labeled tag.

GBA/Glucosylceramidase Protein functions as a critical enzyme within the lysosomal compartment, catalyzing the hydrolysis of glucosylceramides/GlcCers, such as beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine, into free ceramides, such as N-acylsphing-4-enine, and glucose. This enzymatic process is central to the degradation of complex lipids and the turnover of cellular membranes. Additionally, GBA plays a role in the PKC-activated salvage pathway of ceramide formation through the production of ceramides. The protein exhibits transglucosylation activity, facilitating the glucosylation of cholesterol by transferring glucose from GlcCer to cholesterol. It shows a preference for GlcCer containing mono-unsaturated fatty acids as glucose donors for cholesterol glucosylation. Under specific conditions, GBA may catalyze the reverse reaction, transferring glucose from cholesteryl 3-beta-D-glucoside to ceramide, or hydrolyze cholesteryl 3-beta-D-glucoside, producing glucose and cholesterol. Additionally, GBA can hydrolyze galactosylceramides/GalCers, such as beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine, and transfer galactose between GalCers and cholesterol, albeit with lower activity than with GlcCers. Notably, xylosylceramide/XylCer does not serve as a good substrate for hydrolysis, but it acts as a proficient xylose donor for transxylosylation activity to form cholesteryl 3-beta-D-xyloside.

Mouse

HEK293

N-10*His

P17439 (A20-Q515)

14466

AQPCIPKSFGYSSVVCVCNASYCDSLDPVTLPALGTFSRYESTRRGRRMELSVGAIQANRTGTGLLLTLQPEKKFQKVKGFGGAMTDATALNILALSPPTQKLLLRSYFSTNGIEYNIIRVPMASCDFSIRVYTYADTPNDFQLSNFSLPEEDTKLKIPLIHQALKMSSRPISLFASPWTSPTWLKTNGRVNGKGSLKGQPGDIFHQTWANYFVKFLDAYAKYGLRFWAVTAENEPTAGLFTGYPFQCLGFTPEHQRDFISRDLGPALANSSHDVKLLMLDDQRLLLPRWAEVVLSDPEAAKYVHGIAVHWYMDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVTGWTDWNLALNPEGGPNWVRNFVDSPIIVDIPKDAFYKQPMFYHLGHFSKFIPEGSQRVALVASESTDLETVALLRPDGSAVVVVLNRSSEDVPLTISDPDLGFLETVSPGYSIHTYLWRRQ

Approximately 67 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1.0 EU/μg, determined by LAL method.