1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. GCDH Protein, Human (His)

The GCDH protein is essential for the degradation of L-lysine, L-hydroxylysine, and L-tryptophan. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA, releasing CO(2). GCDH Protein, Human (His) is the recombinant human-derived GCDH protein, expressed by E. coli , with N-6*His labeled tag.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

The GCDH protein is essential for the degradation of L-lysine, L-hydroxylysine, and L-tryptophan. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA, releasing CO(2). GCDH Protein, Human (His) is the recombinant human-derived GCDH protein, expressed by E. coli , with N-6*His labeled tag.

Background

GCDH Protein plays a crucial role in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism by catalyzing the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA, accompanied by the release of CO(2). This enzymatic process involves the utilization of electron transfer flavoprotein as its electron acceptor, highlighting the protein's dependence on this cofactor for efficient functioning within the metabolic pathway. It's noteworthy that the Short isoform of GCDH Protein is inactive, indicating the existence of multiple isoforms with varying functional properties in the regulation of this metabolic process.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

E. coli

Tag

N-6*His

Accession

Q92947-1 (R45-K438)

Gene ID
Molecular Construction
N-term
6*His
GCDH (R45-K438)
Accession # Q92947-1
C-term
Synonyms
Glutaryl-CoA Dehydrogenase Mitochondrial; GCD; GCDH
AA Sequence

RPEFDWQDPLVLEEQLTTDEILIRDTFRTYCQERLMPRILLANRNEVFHREIISEMGELGVLGPTIKGYGCAGVSSVAYGLLARELERVDSGYRSAMSVQSSLVMHPIYAYGSEEQRQKYLPQLAKGELLGCFGLTEPNSGSDPSSMETRAHYNSSNKSYTLNGTKTWITNSPMADLFVVWARCEDGCIRGFLLEKGMRGLSAPRIQGKFSLRASATGMIIMDGVEVPEENVLPGASSLGGPFGCLNNARYGIAWGVLGASEFCLHTARQYALDRMQFGVPLARNQLIQKKLADMLTEITLGLHACLQLGRLKDQDKAAPEMVSLLKRNNCGKALDIARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEGTHDIHALILGRAITGIQAFTASK

Molecular Weight

Approximately 41.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM HEPES, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

GCDH Protein, Human (His) Related Classifications

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Inquiry Information

Product Name:
GCDH Protein, Human (His)
Cat. No.:
HY-P70888
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