GCDH Protein, Human (His)

The GCDH protein is essential for the degradation of L-lysine, L-hydroxylysine, and L-tryptophan. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA, releasing CO(2). GCDH Protein, Human (His) is the recombinant human-derived GCDH protein, expressed by E. coli , with N-6*His labeled tag.

GCDH Protein plays a crucial role in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism by catalyzing the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA, accompanied by the release of CO(2). This enzymatic process involves the utilization of electron transfer flavoprotein as its electron acceptor, highlighting the protein's dependence on this cofactor for efficient functioning within the metabolic pathway. It's noteworthy that the Short isoform of GCDH Protein is inactive, indicating the existence of multiple isoforms with varying functional properties in the regulation of this metabolic process.

Human

E. coli

N-6*His

Q92947-1 (R45-K438)

2639  [NCBI]

RPEFDWQDPLVLEEQLTTDEILIRDTFRTYCQERLMPRILLANRNEVFHREIISEMGELGVLGPTIKGYGCAGVSSVAYGLLARELERVDSGYRSAMSVQSSLVMHPIYAYGSEEQRQKYLPQLAKGELLGCFGLTEPNSGSDPSSMETRAHYNSSNKSYTLNGTKTWITNSPMADLFVVWARCEDGCIRGFLLEKGMRGLSAPRIQGKFSLRASATGMIIMDGVEVPEENVLPGASSLGGPFGCLNNARYGIAWGVLGASEFCLHTARQYALDRMQFGVPLARNQLIQKKLADMLTEITLGLHACLQLGRLKDQDKAAPEMVSLLKRNNCGKALDIARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEGTHDIHALILGRAITGIQAFTASK

Approximately 41.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.