GPNMB/Osteoactivin Protein, Human (HEK293, His)

GPNMB, also known as osteoactivin, is a protein thought to have a potential role as a melanin-producing enzyme. Melanogenic enzymes are involved in the production of melanin, the pigment that determines skin, hair and eye color. GPNMB/Osteoactivin Protein, Human (HEK293, His) is the recombinant human-derived GPNMB/Osteoactivin protein, expressed by HEK293 , with C-6*His labeled tag.

GPNMB, also referred to as Osteoactivin, is a protein that has been proposed to have a potential role as a melanogenic enzyme. Melanogenic enzymes are involved in the production of melanin, the pigment responsible for skin, hair, and eye color. While the exact mechanism of action of GPNMB in melanin synthesis is not fully understood, studies have suggested its involvement in this process. Further research is needed to elucidate the specific functions and regulatory mechanisms of GPNMB in melanogenesis and to determine its significance in melanin production.

Measured in a cell proliferation assay using HUVEC cells. The ED₅₀ this effect is 1.704 μg/mL, corresponding to a specific activity is 5.86×10³ units/mg.

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  Measured in a cell proliferation assay using HUVEC cells. The ED₅₀ for this effect is 1.704 μg/mL, corresponding to a specific activity is 5.86×10³ units/mg.

Human

HEK293

C-6*His

Q14956-1 (A22-P486)

10457  [NCBI]

AKRFHDVLGNERPSAYMREHNQLNGWSSDENDWNEKLYPVWKRGDMRWKNSWKGGRVQAVLTSDSPALVGSNITFAVNLIFPRCQKEDANGNIVYEKNCRNEAGLSADPYVYNWTAWSEDSDGENGTGQSHHNVFPDGKPFPHHPGWRRWNFIYVFHTLGQYFQKLGRCSVRVSVNTANVTLGPQLMEVTVYRRHGRAYVPIAQVKDVYVVTDQIPVFVTMFQKNDRNSSDETFLKDLPIMFDVLIHDPSHFLNYSTINYKWSFGDNTGLFVSTNHTVNHTYVLNGTFSLNLTVKAAAPGPCPPPPPPPRPSKPTPSLATTLKSYDSNTPGPAGDNPLELSRIPDENCQINRYGHFQATITIVEGILEVNIIQMTDVLMPVPWPESSLIDFVVTCQGSIPTEVCTIISDPTCEITQNTVCSPVDVDEMCLLTVRRTFNGSGTYCVNLTLGDDTSLALTSTLISVP

80-120 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.