HEXB/Hexosaminidase B Protein, Mouse (HEK293, His)

HEXB, or Hexosaminidase B protein, hydrolyzes N-acetyl-D-hexosamine in glycoconjugates. It selectively acts on neutral oligosaccharides, not degrading other substrates. HEXB does not degrade GM2 gangliosides; only isozyme A does. During fertilization, HEXB contributes to the zona block to polyspermy by inactivating the sperm galactosyltransferase-binding site. This mechanism prevents sperm binding to the zona pellucida. HEXB/Hexosaminidase B Protein, Mouse (HEK293, His) is the recombinant mouse-derived HEXB/Hexosaminidase B protein, expressed by HEK293 , with C-His labeled tag. The total length of HEXB/Hexosaminidase B Protein, Mouse (HEK293, His) is 505 a.a., with molecular weight of ~59.4 kDa.

HEXB, or Hexosaminidase B protein, plays a crucial role in hydrolyzing the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine found in glycoconjugates. This includes oligosaccharide moieties from proteins, neutral glycolipids, and certain mucopolysaccharides. Interestingly, isozyme B exhibits selective substrate specificity, efficiently hydrolyzing neutral oligosaccharides while not acting on other substrates. It is important to note that only isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A. Additionally, during fertilization, HEXB contributes to the zona block to polyspermy, being present in the cortical granules of non-activated oocytes. Upon oocyte activation, HEXB is exocytosed during the cortical reaction, effectively inactivating the sperm galactosyltransferase-binding site. This molecular mechanism accounts for the block in sperm binding to the zona pellucida, as reported in studies.

Measured by its ability to hydrolyze 4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide (4-MU-GlcNAc). Read at excitation and emission wavelengths of 365 nm and 445 nm (top read). The specific activity is 3033.81 pmol/min/µg, as measured under the described conditions.

Mouse

HEK293

C-His

P20060 (Q32-I536)

15212  [NCBI]

QPALWPFPRSVQMFPRLLYISAEDFSIDHSPNSTAGPSCSLLQEAFRRYYNYVFGFYKRHHGPARFRAEPQLQKLLVSITLESECESFPSLSSDETYSLLVQEPVAVLKANSVWGALRGLETFSQLVYQDSFGTFTINESSIADSPRFPHRGILIDTSRHFLPVKTILKTLDAMAFNKFNVLHWHIVDDQSFPYQSTTFPELSNKGSYSLSHVYTPNDVRMVLEYARLRGIRVIPEFDTPGHTQSWGKGQKNLLTPCYNQKTKTQVFGPVDPTVNTTYAFFNTFFKEISSVFPDQFIHLGGDEVEFQCWASNPNIQGFMKRKGFGSDFRRLESFYIKKILEIISSLKKNSIVWQEVFDDKVELQPGTVVEVWKSEHYSYELKQVTGSGFPAILSAPWYLDLISYGQDWKNYYKVEPLNFEGSEKQKQLVIGGEACLWGEFVDATNLTPRLWPRASAVGERLWSPKTVTDLENAYKRLAVHRCRMVSRGIAAQPLYTGYCNYENKI

Approximately 59.4 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.