gp140 Protein, HIV-1 (627a.a, HEK293, Fc)

gp140 is a non-cleaved exodomain of (gp160)3, with both its transmembrane segment (TM) and cytoplasmic tail (CT) truncated, and is often considered a possible replacement for natural Env spikes.However, most HIV-1 gp140 preparations are unstable and have an uneven conformation.gp140 Protein, HIV-1 (HEK293, Fc) is the recombinant Virus-derived gp140 protein, expressed by HEK293 , with C-hFc labeled tag.

HIV-1 包膜突刺 三聚体(gp160)3, cleaved to (gp120/gp41)3 是一种 I 型膜蛋白，它融合病毒和宿主细胞膜，引发病毒感染。gp140 是 (gp160)3 的非切割外结构域，其跨膜段 (TM) 和细胞质尾部 (CT) 都被截断，通常被认为是天然 Env 尖峰的可能替代物。然而，大多数 HIV-1 gp140 制剂不稳定且构象不均匀。

Virus

HEK293

C-hFc

AAC97548.1 (L33-K659)

/

LWVTVYYGVPIWKDANTTLFCASDAKAYDTEVHNVWATHACVPTDPSPQELKMENVTEEFNMWKNNMVEQMHTDIISLWDQSLKPCVQLTPLCVTLDCSYNITNNITNSITNSSVNMREEIKNCSFNMTTELRDKNRKVYSLFYKLDVVQINNGNNSSNLYRLINCNTSALTQARPKVTFEPIPIHYCAPAGYAILKCNDKEFNGTGLCKNVSTVQCTHGIRPVVSTQLLLNGSLAEGKVMIRSENITNNVKNIIVQLNESVTINCTRPNNNTRRSVRIGPGQTFYATGDIIGDIRQAHCNVSGSQWNKTLHQVVEQLRKYWNNNTIIFNSSSGGDLEITTHSFNCAGEFFYCNTSGLFNSTWVNGTTSSTSNGTITLPCRIKQIINMWQRVGQAMYAPPIQGVIKCESNITGLILTRDGGVNSSDSETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKARRRVVEREKRAVTLGAVFIGFLGTAGSTMGAASITLTVQARKLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICPTNVPWNSSWSNKSLDEIWENMTWLQWDKEISNYTIKIYELIEESQIQQERNEKDLLELDK

Approximately 97.4 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.