HNRNPH1 Protein, Human (His-SUMO)

The HNRNPH1 protein is an important component of the hnRNP complex and plays a key role in pre-mRNA processing events leading to mRNA conversion in the cytoplasm. Functionally, it cooperates with CUGBP1 to regulate alternative splicing of pre-mRNA, specifically inhibiting the inclusion of insulin receptor (IR) pre-mRNA exon 11 in myoblasts. HNRNPH1 Protein, Human (His-SUMO) is the recombinant human-derived HNRNPH1 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of HNRNPH1 Protein, Human (His-SUMO) is 448 a.a., with molecular weight of ~65.1 kDa.

The HNRNPH1 protein plays a crucial role as a component of heterogeneous nuclear ribonucleoprotein (hnRNP) complexes, pivotal entities in the processing events that precede the functional transformation of pre-mRNAs into translatable mRNAs in the cytoplasm. Functionally, HNRNPH1 mediates the regulation of pre-mRNA alternative splicing, with notable inhibitory effects on insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblasts, a process it accomplishes in collaboration with CUGBP1. HNRNPH1 demonstrates a specific binding affinity for IR RNA and poly(RG), and it forms part of a ternary complex alongside FUBP2, PTBP1, and PTBP2. Additionally, HNRNPH1 is identified in the spliceosome C complex and interacts with IGF2BP1, CUGBP1 (in an RNA-dependent manner), and MBNL1 (in an RNA-independent manner). These interactions underscore the multifaceted and dynamic role of HNRNPH1 in orchestrating pre-mRNA processing and alternative splicing, contributing to the intricate regulation of gene expression.

Human

E. coli

N-6*His;N-SUMO

P31943 (M2-A449)

3187  [NCBI]

MLGTEGGEGFVVKVRGLPWSCSADEVQRFFSDCKIQNGAQGIRFIYTREGRPSGEAFVELESEDEVKLALKKDRETMGHRYVEVFKSNNVEMDWVLKHTGPNSPDTANDGFVRLRGLPFGCSKEEIVQFFSGLEIVPNGITLPVDFQGRSTGEAFVQFASQEIAEKALKKHKERIGHRYIEIFKSSRAEVRTHYDPPRKLMAMQRPGPYDRPGAGRGYNSIGRGAGFERMRRGAYGGGYGGYDDYNGYNDGYGFGSDRFGRDLNYCFSGMSDHRYGDGGSTFQSTTGHCVHMRGLPYRATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEDAVAAMSKDKANMQHRYVELFLNSTAGASGGAYEHRYVELFLNSTAGASGGAYGSQMMGGMGLSNQSSYGGPASQQLSGGYGGGYGGQSSMSGYDQVLQENSSDFQSNIA

Approximately 65.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.