1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-17
  5. IL-17A IL-17F
  6. IL-17A/F Heterodimer Protein, Rhesus Macaque (HEK293, His)

IL-17A/F Heterodimer Protein, Rhesus Macaque (HEK293, His)

Cat. No.: HY-P74832
Handling Instructions Technical Support

IL-17A-17F Heterodimer Protein is the heterodimer of the cytokines IL-17A and IL-17F. Both IL-17A and IL-17F can induce antimicrobial peptides, cytokines (IL-6 and GM-CSF), chemokines (CCL2, CCL7 and CXCL1), and matrix metalloproteinases (MMP-1 and MMP13). IL-17A-17F shows intermediate biological activity between IL-17A and IL-17F.IL-17A/F Heterodimer Protein, Rhesus Macaque (HEK293, His) is a recombinant rhesus macaque IL-17A-17F heterodimer protein with His tag at the C-terminus and is expressed in HEK293 cells.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

IL-17A-17F Heterodimer Protein is the heterodimer of the cytokines IL-17A and IL-17F. Both IL-17A and IL-17F can induce antimicrobial peptides, cytokines (IL-6 and GM-CSF), chemokines (CCL2, CCL7 and CXCL1), and matrix metalloproteinases (MMP-1 and MMP13). IL-17A-17F shows intermediate biological activity between IL-17A and IL-17F[1][2][3][4].IL-17A/F Heterodimer Protein, Rhesus Macaque (HEK293, His) is a recombinant rhesus macaque IL-17A-17F heterodimer protein with His tag at the C-terminus and is expressed in HEK293 cells.

Background

IL-17A-17F Heterodimer Protein is the heterodimer of the cytokines IL-17A and IL-17F. Both IL-17A and IL-17F belongs to the IL-17 cytokine family. IL-17A-17F heterodimer, IL-17A and IL-17F homodimers can be produced by differentiated Th17 cells[1][2]. IL-17F shares the most similarities with IL-17A (50% homology)[2]. Both IL-17A and IL-17F can induces antimicrobial peptides, cytokines (IL-6 and GM-CSF), chemokines (CCL2, CCL7 and CXCL1), and matrix metalloproteinases (MMP-1 and MMP13)[2][3]. IL-17A, IL-17F and IL-17A-17F use the same receptor complex: IL-17RA and IL-17RC heterodimer. And they trigger qualitatively similar signaling pathways. IL-17A-17F shows intermediate biological activity between IL-17A (most potent) and IL-17F (least potent)[2][4].

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized Rhesus Macaque IL-17A-17F at 2 μg/ml (100 μl/well) can bind Rhesus Macaque IL-17RA, The ED50 for this effect is 8.193 ng/mL.

Species

Rhesus Macaque

Source

HEK293

Tag

C-6*His

Accession

XP_001106391 (G24-A155)&NP_001248216 (R31-Q163)

Gene ID
Molecular Construction
N-term
IL-17A (G24-A155)
Accession # XP_001106391
6*His
C-term
N-term
IL-17F (R31-Q163)
Accession # NP_001248216
6*His
C-term
Synonyms
CTLA-8; IL-17; Interleukin-17A; IL17A; Interleukin-17F; IL-17F; Cytokine ML-1
Molecular Weight

Predict MW: 15.94 & 15.78 kDa; Approximately 17 & 24 kDa, respectively, corresponding to the molecualr weight of 17F and 17A, in SDS-PAGE under reducing conditions, due to the glycosylation.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

IL-17A/F Heterodimer Protein, Rhesus Macaque (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-17A/F Heterodimer Protein, Rhesus Macaque (HEK293, His)
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