2. Recombinant Proteins
  3. Cytokines and Growth Factors CD Antigens
  4. Interleukin & Receptors Monocyte CD Proteins
  5. IL-1R IL-1R2/CD121b
  6. IL-1R2/CD121b
  7. IL-1R2 Protein, Mouse (HEK293, hFc)

IL-1R2 is a competitive endogenous inhibitor of IL-1 signaling. IL-1R2 serves as a decoy receptor and competes with IL-1R1 for IL-1, it can also form a complex with IL-1RAP once it binds IL-1. IL-1R2 is implicated in various IL-1-mediated inflammatory diseases. IL-1R2 Protein, Mouse (HEK293, hFc) is a recombinant mouse IL-1R2 protein with an hFc tag at the C-terminus and is expressed in HEK293 cells. It consists of 355 amino acids (M1-E355).

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Description

IL-1R2 is a competitive endogenous inhibitor of IL-1 signaling. IL-1R2 serves as a decoy receptor and competes with IL-1R1 for IL-1, it can also form a complex with IL-1RAP once it binds IL-1. IL-1R2 is implicated in various IL-1-mediated inflammatory diseases[1]. IL-1R2 Protein, Mouse (HEK293, hFc) is a recombinant mouse IL-1R2 protein with an hFc tag at the C-terminus and is expressed in HEK293 cells. It consists of 355 amino acids (M1-E355).
Background

IL-1R2 is the non-signaling type 2 interleukin-1 receptor and is expressed in DC, CD4+ T cells, T regulatory cells, monocytes, neutrophils and different macrophagic cells in mouse. The mouse IL-1R2 can be cleaved into two forms: membrane form (14-410 a.a) and soluble form (14-? a.a).
The sequence of amino acids in IL-1R2 from mouse shows low similarity (about 60%) with human IL-1R2, but has been only slightly altered with rat IL-1R2 (90 % similarity).
IL-1R2 contains truncated cytoplasmic domain and lacks Toll-IL-1 receptor (TIR) region, making it incapable of transmembrane signaling. IL-1R2 serves as an endogenous inhibitor of IL-1 signaling. Functional IL-1 signaling requires IL-1R1 and IL-1-dependent recruitment of IL-1RAP. IL-1R2 serves as a decoy receptor and can compete with IL-1R1 for IL-1. IL-1R2 can also form a complex with IL-1RAP once it binds IL-1, preventing IL-1RAP from heterodimerizing with IL-1R1. Through these 2 ways, IL-1R2 blocks IL-1 signaling. Additionally, soluble IL-1R2 recruits soluble IL-1RAP with high affinity without impacting affinity for IL-1RA.
IL-1R2 inhibits IL-1 signaling and has been implicated in various IL-1-mediated inflammatory diseases like arthritis, diabetes, gout and so on[1][2][3].
In Vitro

IL-1R2 (24 h) specifically inhibits IL-1β-induced urokinase-type plasminogen activator responses in HUVECs[4].
In Vivo

IL-1R2 knockout mice exacerbates cardiac damage in myocardial ischemia reperfusion (I/R) injury and knockout of IL-1R2 induces cytokines expression in the heart after I/R injury[3].
AAV9-IL-1R2 (4 µL; myocardial injection, once) protects heart against IL-17A-induced cardiomyocytes apoptosis through suppressing IL-17RA expression in mice[3].
Species

Mouse
Source

HEK293
Tag

C-hFc
Accession

P27931 (F14-E355)
Gene ID
Molecular Construction
N-term
IL-1R2 (F14-E355)
Accession # P27931
hFc
C-term
Synonyms
Interleukin-1 receptor type 2; IL-1R-2; CDw121b; IL-1R-beta; CD121b; mIL-1R2; sIL-1R2
Molecular Weight

Approximately 91 kDa
Purity

Greater than 85% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Data Sheet (261 KB) Handling Instructions (2659 KB)
References

IL-1R2 Protein, Mouse (HEK293, hFc) Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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