IL-2 Protein, Mouse

IL-2 Protein, Mouse is an immunomodulatory cytokine with widely varying functions including promotion of apoptosis, proliferation and survival of lymphocytes.

Interleukin-2 (IL-2) is a multi-faceted cytokine, known for promoting proliferation, survival, and cell death depending on the cell type and state. For example, IL-2 facilitates cell death only in activated T cells when antigen and IL-2 are abundant. The availability of IL-2 clearly impacts this process. IL-2 is retained in blood vessels by heparan sulfate, and that biologically active IL-2 is released from vessel tissue by heparanase. The morphologic changes and rapid cell death induced by dimeric IL-2 imply that cell death is mediated by disruption of membrane permeability and subsequent necrosis. IL-2 has a direct and unexpectedly broad influence on cellular homeostatic mechanisms in both immune and non-immune systems^[1].

1.Measured in a cell proliferation assay using CTLL-2 Cells. The ED₅₀ for this effect is ≤0.5117 ng/mL, corresponding to a specific activity is 1.954×10⁶ units/mg.
2.Measured in a cell proliferation assay using CTLL 2 mouse cytotoxic T cells.The specific activity of Recombinant Mouse IL-2 is ≥1×10⁷ IU/mg.

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  Measured in a cell proliferation assay using CTLL-2 Cells. The ED₅₀ for this effect is 0.5117 ng/mL, corresponding to a specific activity is 1.954×10⁶ units/mg.

Mouse

E. coli

Tag Free

P04351 (A21-Q169)

16183  [NCBI]

APTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ

Approximately 17 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM Sodium Citrate, 0.2% Tween 80, pH 3.0 or 10 mM Sodium Citrate, pH 4.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Wrenshall LE, et al. Identification of a cytotoxic form of dimeric interleukin-2 in murine tissues. PLoS One. 2014 Jul 14;9(7):e102191.  [Content Brief]