IL-21 Protein, Mouse (Biotinylated, His-Avi)

IL-21 is a cytokine with immunomodulatory capabilities that orchestrates the transition from innate to adaptive immunity. It induces the production of IgG(1) and IgG(3) by B cells, thereby forming a humoral immune response. IL-21 Protein, Mouse (Biotinylated, His-Avi) is the recombinant mouse-derived IL-21 protein, expressed by E. coli , with N-Avi, N-His labeled tag. The total length of IL-21 Protein, Mouse (Biotinylated, His-Avi) is 129 a.a., with molecular weight of ~17.9 kDa.

IL-21, a cytokine endowed with immunoregulatory prowess, emerges as a key player in orchestrating the delicate transition between innate and adaptive immunity. This multifaceted regulator takes the stage in inducing the production of IgG(1) and IgG(3) in B-cells, thereby substantiating its pivotal role in shaping humoral immune responses. IL-21 finds its niche in fostering the generation and perpetuation of T follicular helper (Tfh) cells and the intricate formation of germinal centers. Collaborating synergistically with IL6, it exercises command over the early development of Tfh cells, contributing indispensably to a robust antibody response during acute viral infections. Beyond B-cells, IL-21 extends its influence to the realm of natural killer (NK) cells, where, in synergy with IL15, it fuels their proliferation and maturation. Further, IL-21 flexes its regulatory muscle, guiding the proliferation and maturation of mature B- and T-cells in response to activating stimuli. In tandem with IL15 and IL18, it orchestrates the production of interferon-gamma in both T-cells and NK cells. Notably, during T-cell-mediated immune responses, IL-21 steps in as a potential inhibitor, dampening the activation and maturation of dendritic cells, unveiling a nuanced regulatory role in immune dynamics.

Immobilized Human IL-21R, hFc Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Mouse IL-21, His Tag with the EC₅₀ of ≤6 ng/mL determined by ELISA.

Mouse

E. coli

N-Avi;N-His

Q9ES17 (H18-S146)

60505  [NCBI]

18-25 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 20 mM NaAc, 150 mM NaCl, pH 5.5.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.