Klotho beta Protein, Human (HEK293, His)

Klotho beta protein plays a critical role in the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), a key enzyme controlling bile acid synthesis. Although its glycosidase activity may be inactive, Klotho beta enhances the binding ability of FGFR1 and FGFR4 to FGF21, promoting their interaction. Klotho beta Protein, Human (HEK293, His) is the recombinant human-derived Klotho beta protein, expressed by HEK293 , with C-His labeled tag. The total length of Klotho beta Protein, Human (HEK293, His) is 954 a.a., with molecular weight of 112-140 kDa.

The Klotho beta protein plays a significant role in transcriptional regulation by contributing to the repression of cholesterol 7-alpha-hydroxylase (CYP7A1), a key enzyme in bile acid synthesis. While likely inactive as a glycosidase, Klotho beta enhances the binding ability of FGFR1 and FGFR4 to FGF21, indicating its involvement in fibroblast growth factor (FGF) signaling. The protein directly interacts with both FGF19 and FGF21, underscoring its association with crucial components of the FGF pathway. These interactions, particularly with FGF21, suggest a potential role in metabolic regulation and highlight Klotho beta's involvement in modulating cellular responses and metabolic pathways (

1.Immobilized Human Beta Klotho at 2 μg/mL (100 μL/Well). Dose response curve for Anti-Beta Klotho Antibody1 with the EC₅₀ is < 80.2 ng/mL determined by ELISA.
2.Immobilized Human Beta Klotho at 2 μg/mL (100μl/Well). Dose response curve for Anti-Beta Klotho Antibody2, hFc Tag with the EC₅₀ of < 89.8 ng/ml determined by ELISA.
3.Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.2198 ng/mL in the presence of 1μg/mL Human FGF-21, corresponding to a specific activity is 4.549×10³ units/mg.

Human

HEK293

C-His

Q86Z14 (M30-T983)

152831  [NCBI]

Approximately 112-140 kDa due to the glycosylation.

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.