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  5. LAG-3 Protein, Mouse (419a.a, HEK293, His)

LAG-3 Protein, Mouse (419a.a, HEK293, His)

Cat. No.: HY-P78720
Data Sheet SDS COA Handling Instructions

LAG-3 (lymphocyte activation gene 3) protein is expressed on antigen-activated T cells and transmits inhibitory signals by binding to ligands such as FGL1.FGL1 is the main ligand responsible for the suppressive function of LAG3 T cells.LAG-3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived LAG-3 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

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Description

LAG-3 (lymphocyte activation gene 3) protein is expressed on antigen-activated T cells and transmits inhibitory signals by binding to ligands such as FGL1.FGL1 is the main ligand responsible for the suppressive function of LAG3 T cells.LAG-3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived LAG-3 protein, expressed by HEK293 , with C-His labeled tag.

Background

LAG-3 (Lymphocyte activation gene 3) protein is an inhibitory receptor expressed on antigen-activated T-cells, delivering inhibitory signals upon binding to ligands such as FGL1. Serving as a major ligand of LAG3, FGL1 is responsible for LAG3's T-cell inhibitory function. Following T-cell receptor (TCR) engagement, LAG3 associates with CD3-TCR in the immunological synapse, directly inhibiting T-cell activation. LAG3 may synergistically inhibit antigen-specific T-cell activation with PDCD1/PD-1, possibly acting as a coreceptor for PD-1. It negatively regulates the proliferation, activation, effector function, and homeostasis of both CD8(+) and CD4(+) T-cells. Constitutively expressed on a subset of regulatory T-cells (Tregs), LAG3 contributes to their suppressive function, mediating immune tolerance. Additionally, LAG3 acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation and binds to MHC class II (MHC-II), possibly functioning as a ligand for MHC-II on antigen-presenting cells, thereby promoting APC activation/maturation and driving Th1 immune responses.

Biological Activity

1.Immobilized Mouse LAG-3, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse FGL1. The ED50 for this effect is 0.5983 μg/mL.
2.Immobilized Mouse LAG3 at 5 μ g/mL can bind Human FGL1. The EC50 is 309.8-461.1 ng/mL.

  • Immobilized Mouse LAG-3, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse FGL1. The ED50 for this effect is 0.5983 μg/mL.
Species

Mouse

Source

HEK293

Tag

C-His

Accession

Q61790 (G24-L442)

Gene ID
Molecular Construction
N-term
LAG-3 (G24-L442)
Accession # Q61790
His
C-term
Synonyms
Lymphocyte activation gene 3 protein; LAG3; LAG-3; Protein FDC; CD223
AA Sequence

GPGKELPVVWAQEGAPVHLPCSLKSPNLDPNFLRRGGVIWQHQPDSGQPTPIPALDLHQGMPSPRQPAPGRYTVLSVAPGGLRSGRQPLHPHVQLEERGLQRGDFSLWLRPALRTDAGEYHATVRLPNRALSCSLRLRVGQASMIASPSGVLKLSDWVLLNCSFSRPDRPVSVHWFQGQNRVPVYNSPRHFLAETFLLLPQVSPLDSGTWGCVLTYRDGFNVSITYNLKVLGLEPVAPLTVYAAEGSRVELPCHLPPGVGTPSLLIAKWTPPGGGPELPVAGKSGNFTLHLEAVGLAQAGTYTCSIHLQGQQLNATVTLAVITVTPKSFGLPGSRGKLLCEVTPASGKERFVWRPLNNLSRSCPGPVLEIQEARLLAERWQCQLYEGQRLLGATVYAAESSSGAHSARRISGDLKGGHL

Molecular Weight

53-80 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4 or 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 or PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

LAG-3 Protein, Mouse (419a.a, HEK293, His) Related Classifications

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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LAG-3 Protein, Mouse (419a.a, HEK293, His)
Cat. No.:
HY-P78720
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