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  4. MG281 Protein, Mycoplasma genitalium (Cell-Free, His)

MG281 Protein, Mycoplasma genitalium (Cell-Free, His)

Cat. No.: HY-P702378
Handling Instructions

MG281 Protein, Mycoplasma genitalium (Cell-Free, His) is the recombinant MG281 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of MG281 Protein, Mycoplasma genitalium (Cell-Free, His) is 556 a.a., with molecular weight of 65.0 kDa.

For research use only. We do not sell to patients.

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Description

MG281 Protein, Mycoplasma genitalium (Cell-Free, His) is the recombinant MG281 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of MG281 Protein, Mycoplasma genitalium (Cell-Free, His) is 556 a.a., with molecular weight of 65.0 kDa.
Species

Others
Source

E. coli Cell-free
Tag

N-10*His
Accession

P47523 (M1-L556)
Gene ID

/
Molecular Construction
N-term
10*His
MG281 (M1-L556)
Accession # P47523
C-term
Synonyms
Uncharacterized protein MG281
AA Sequence

MQFKKHKNSVKFKRKLFWTIGVLGAGALTTFSAVMITNLVNQSGYALVASGRSGNLGFKLFSTQSPSAEVKLKSLSLNDGSYQSEIDLSGGANFREKFRNFANELSEAITNSPKGLDRPVPKTEISGLIKTGDNFITPSFKAGYYDHVASDGSLLSYYQSTEYFNNRVLMPILQTTNGTLMANNRGYDDVFRQVPSFSGWSNTKATTVSTSNNLTYDKWTYFAAKGSPLYDSYPNHFFEDVKTLAIDAKDISALKTTIDSEKPTYLIIRGLSGNGSQLNELQLPESVKKVSLYGDYTGVNVAKQIFANVVELEFYSTSKANSFGFNPLVLGSKTNVIYDLFASKPFTHIDLTQVTLQNSDNSAIDANKLKQAVGDIYNYRRFERQFQGYFAGGYIDKYLVKNVNTNKDSDDDLVYRSLKELNLHLEEAYREGDNTYYRVNENYYPGASIYENERASRDSEFQNEILKRAEQNGVTFDENIKRITASGKYSVQFQKLENDTDSSLERMTKAVEGLVTVIGEEKFETVDITGVSSDTNEVKSLAKELKTNALGVKLKL

Molecular Weight

65.0 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

MG281 Protein, Mycoplasma genitalium (Cell-Free, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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