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  2. Enzymes & Regulators
  3. MVK Protein, Human (sf9, His-GST)

MVK Protein, Human (sf9, His-GST)

Cat. No.: HY-P74739
COA Handling Instructions

MVK (mevalonate kinase) protein plays a vital role in cellular metabolism by catalyzing the phosphorylation of mevalonate to mevalonate 5-phosphate, which is responsible for isoprenoid and cholesterol biosynthesis. key step. MVK regulates pathways necessary for the synthesis of important cellular components. MVK Protein, Human (sf9, His-GST) is the recombinant human-derived MVK protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of MVK Protein, Human (sf9, His-GST) is 396 a.a., with molecular weight of ~47 kDa.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
5 μg $125 In-stock
10 μg $214 In-stock
50 μg   Get quote  
100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

MVK (mevalonate kinase) protein plays a vital role in cellular metabolism by catalyzing the phosphorylation of mevalonate to mevalonate 5-phosphate, which is responsible for isoprenoid and cholesterol biosynthesis. key step. MVK regulates pathways necessary for the synthesis of important cellular components. MVK Protein, Human (sf9, His-GST) is the recombinant human-derived MVK protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of MVK Protein, Human (sf9, His-GST) is 396 a.a., with molecular weight of ~47 kDa.

Background

The MVK protein plays a pivotal role in isoprenoid and cholesterol biosynthesis by catalyzing the phosphorylation of mevalonate to mevalonate 5-phosphate. This enzymatic activity represents a crucial and rate-limiting step in the mevalonate pathway, a metabolic pathway essential for the synthesis of various important molecules, including cholesterol and isoprenoid compounds. The phosphorylation of mevalonate by MVK is a tightly regulated process and a key regulatory point in the overall control of cholesterol and isoprenoid production within cells. Dysregulation of MVK activity has been associated with certain metabolic disorders, emphasizing the significance of this enzyme in maintaining cellular homeostasis and lipid metabolism.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

Sf9 insect cells

Tag

N-His;N-GST

Accession

Q03426 (M1-L396)

Gene ID
Molecular Construction
N-term
His-GST
MVK (M1-L396)
Accession # Q03426
C-term
Synonyms
Mevalonate kinase; MK; MVK
Molecular Weight

Approximately 47 kDa

Purity

Greater than 85% as determined by reducing SDS-PAGE

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 2 mM DTT, pH 7.4, 10% gly.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

MVK Protein, Human (sf9, His-GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
MVK Protein, Human (sf9, His-GST)
Cat. No.:
HY-P74739
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