NGFR Protein, Mouse (HEK293, Fc)

Nerve Growth Factor Receptor (NGFR) also known as CD271, p75NTR, TNFRSF16, is a transmembrane low-affinity receptor for the neurotrophin family. NGFR has no intrinsic tyrosine kinase activity. NGFR can act as a tumour suppressor, negatively regulating cell growth and proliferation^[1]. NGF-NGFR cascade activates NF-κB, leading to inhibition of apoptosis, increases survival of schwannoma, and breast cancer cells^[3].  NGFR Protein, Mouse (HEK293, Fc) is a recombinant protein with a C-Terminal Fc label, It consists of 243 amino acids and is produced in HEK293 cells.

Nerve Growth Factor Receptor (NGFR) is expressed not only in nervous tissue, but also in non-neuronal normal and cancer cells, such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells and myoepithelial cells^[1].
Human NGFR shares 92.45% aa sequence identity with mouse NGFR protein and 92.42% aa sequence identity with rat NGFR protein.
Nerve Growth Factor Receptor (NGFR) is a type-I transmembrane protein, a typical structure of the TNFR superfamily and devoid of intrinsic catalytic activity. NGFR signaling involves activation of NF-kB (Rel/NF-kB transcription factors) and the phosphorylation of the transcription factor c-Jun kinase (JNK), as well as increased production of ceramide, leading to gene transcription or programmed cell death^[2].
NGFR induces p53-dependent apoptosis and cell growth arrest as well as suppressed tumor growth^[3]. The low-affinity nerve growth factor receptor (NGFR) p75NGFR induces apoptosis in the absence of nerve growth factor (NGF) binding but enhances neural survival when bound by NGF. NGFR enhances beta-amyloid peptide toxicity^[4]. NGFR signal can induce the subsequent downregulation of melanoma antigens and eventually suppress CTL activation^[5].

Measured by its ability to inhibit NGF-dependent proliferation of TF-1 human erythroleukemic cells.The ED₅₀ for this effect is typically 0.5-3 μg/mL in the presence of 2 ng/mL Recombinant mouse NGF.

Mouse

HEK293

C-hFc

Q9Z0W1 (M1-N243)

18053  [NCBI]

70-80 kDa

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Reis-Filho JS, et al. Distribution and significance of nerve growth factor receptor (NGFR/p75NTR) in normal, benign and malignant breast tissue. Mod Pathol. 2006 Feb;19(2):307-19.  [Content Brief]

  [2]. Micera A, et al. Nerve growth factor and tissue repair remodeling: trkA(NGFR) and p75(NTR), two receptors one fate. Cytokine Growth Factor Rev. 2007 Jun-Aug;18(3-4):245-56.  [Content Brief]

  [3]. Zhou X, et al. Nerve growth factor receptor negates the tumor suppressor p53 as a feedback regulator. Elife. 2016 Jun 10;5:e15099.  [Content Brief]

  [4]. Rabizadeh S, et al. Expression of the low-affinity nerve growth factor receptor enhances beta-amyloid peptide toxicity. Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10703-6.  [Content Brief]

  [5]. Furuta J, et al. CD271 on melanoma cell is an IFN-γ-inducible immunosuppressive factor that mediates downregulation of melanoma antigens. J Invest Dermatol. 2014 May;134(5):1369-1377.  [Content Brief]

  [6]. Chung MK, et al. CD271 Confers an Invasive and Metastatic Phenotype of Head and Neck Squamous Cell Carcinoma through the Upregulation of Slug. Clin Cancer Res. 2018 Feb 1;24(3):674-683.  [Content Brief]