1. Recombinant Proteins
  2. Others
  3. Nicastrin Protein, Mouse (HEK293, His)

Nicastrin Protein, Mouse (HEK293, His)

Cat. No.: HY-P74696
COA Handling Instructions

Nicastrin is an important subunit of the γ-secretase complex and plays a key role in the endoproteolytic cleavage of integral membrane proteins, including Notch receptors and amyloid-β precursor protein (APP). In the γ-secretase complex, nicastin acts as a key regulator of Notch and Wnt signaling cascades and affects downstream processes. Nicastrin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Nicastrin protein, expressed by HEK293 , with C-His labeled tag. The total length of Nicastrin Protein, Mouse (HEK293, His) is 641 a.a., with molecular weight of 95-99 kDa.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
Free Sample   Apply now
5 μg $40 In-stock
10 μg $70 In-stock
50 μg $200 In-stock
100 μg $340 In-stock
> 100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Nicastrin is an important subunit of the γ-secretase complex and plays a key role in the endoproteolytic cleavage of integral membrane proteins, including Notch receptors and amyloid-β precursor protein (APP). In the γ-secretase complex, nicastin acts as a key regulator of Notch and Wnt signaling cascades and affects downstream processes. Nicastrin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Nicastrin protein, expressed by HEK293 , with C-His labeled tag. The total length of Nicastrin Protein, Mouse (HEK293, His) is 641 a.a., with molecular weight of 95-99 kDa.

Background

Nicastrin, an indispensable subunit of the gamma-secretase complex, takes center stage in the intricate molecular machinery responsible for the endoproteolytic cleavage of integral membrane proteins. This includes pivotal substrates like Notch receptors and the amyloid-beta precursor protein (APP). The gamma-secretase complex, in which Nicastrin plays a crucial role, serves as a linchpin in the modulation of Notch and Wnt signaling cascades, exerting regulatory influence on downstream processes. In concert with other essential components, such as a presenilin homodimer, APH1, and PEN2, Nicastrin forms the functional gamma-secretase complex. Nicastrin's binding affinity extends to the proteolytically processed C-terminal fragments of APP, further underscoring its pivotal role in orchestrating the intricacies of this proteolytic machinery. Moreover, Nicastrin's interactions with presenilin components accentuate its integral role within the gamma-secretase complex.

Species

Mouse

Source

HEK293

Tag

C-10*His

Accession

P57716 (G28-E668)

Gene ID
Molecular Construction
N-term
Nicastrin (G28-E668)
Accession # P57716
His
C-term
Synonyms
Nicastrin; NCSTN; KIAA0253
AA Sequence

GLCGGNSVERKIYIPLNKTAPCVRLLNATHQIGCQSSISGDTGVIHVVEKEEDLKWVLTDGPNPPYMVLLEGKLFTRDVMEKLKGTTSRIAGLAVTLAKPNSTSSFSPSVQCPNDGFGIYSNSYGPEFAHCKKTLWNELGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHNLGQNGSAPSFPLCAMQLFSHMHAVISTATCMRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSVGLEPDVRVVVAATRLDSRSFFWNVAPGAESAVASFVTQLAAAEALHKAPDVTTLSRNVMFVFFQGETFDYIGSSRMVYDMENGKFPVRLENIDSFVELGQVALRTSLDLWMHTDPMSQKNESVKNQVEDLLATLEKSGAGVPEVVLRRLAQSQALPPSSLQRFLRARNISGVVLADHSGSFHNRYYQSIYDTAENINVTYPEWQSPEEDLNFVTDTAKALANVATVLARALYELAGGTNFSSSIQADPQTVTRLLYGFLVRANNSWFQSILKHDLRSYLDDRPLQHYIAVSSPTNTTYVVQYALANLTGKATNLTREQCQDPSKVPNESKDLYEYSWVQGPWNSNRTERLPQCVRSTVRLARALSPAFELSQWSSTEYSTWAESRWKDIQARIFLIASKELE

Molecular Weight

Approximately 95-120 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Nicastrin Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Nicastrin Protein, Mouse (HEK293, His)
Cat. No.:
HY-P74696
Quantity:
MCE Japan Authorized Agent: