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  3. OPN1LW Protein, Human (Cell-Free, His)

OPN1LW Protein, Human (Cell-Free, His)

Cat. No.: HY-P702398
Handling Instructions

OPN1LW protein, crucial for vision, is a vital element of visual pigments responsible for light absorption. The pigments consist of opsin, forming a covalent bond with cis-retinal. This molecular mechanism is indispensable for perceiving light stimuli in vision. OPN1LW Protein, Human (Cell-Free, His) is the recombinant human-derived OPN1LW protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of OPN1LW Protein, Human (Cell-Free, His) is 364 a.a., with molecular weight of 43.4 kDa.

For research use only. We do not sell to patients.

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Description

OPN1LW protein, crucial for vision, is a vital element of visual pigments responsible for light absorption. The pigments consist of opsin, forming a covalent bond with cis-retinal. This molecular mechanism is indispensable for perceiving light stimuli in vision. OPN1LW Protein, Human (Cell-Free, His) is the recombinant human-derived OPN1LW protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of OPN1LW Protein, Human (Cell-Free, His) is 364 a.a., with molecular weight of 43.4 kDa.

Background

The OPN1LW protein, integral to the process of vision, is a key component of visual pigments, which are responsible for absorbing light. These pigments comprise an apoprotein known as opsin, which forms a covalent linkage with cis-retinal, facilitating the intricate molecular mechanism essential for the perception of light stimuli in vision.

Species

Human

Source

E. coli Cell-free

Tag

N-10*His

Accession

P04000 (M1-A364)

Gene ID

/

Molecular Construction
N-term
10*His
OPN1LW (M1-A364)
Accession # P04000
C-term
Synonyms
Long-wave-sensitive opsin 1; Red cone photoreceptor pigment; Red-sensitive opsin; ROP
AA Sequence

MAQQWSLQRLAGRHPQDSYEDSTQSSIFTYTNSNSTRGPFEGPNYHIAPRWVYHLTSVWMIFVVTASVFTNGLVLAATMKFKKLRHPLNWILVNLAVADLAETVIASTISIVNQVSGYFVLGHPMCVLEGYTVSLCGITGLWSLAIISWERWLVVCKPFGNVRFDAKLAIVGIAFSWIWSAVWTAPPIFGWSRYWPHGLKTSCGPDVFSGSSYPGVQSYMIVLMVTCCIIPLAIIMLCYLQVWLAIRAVAKQQKESESTQKAEKEVTRMVVVMIFAYCVCWGPYTFFACFAAANPGYAFHPLMAALPAYFAKSATIYNPVIYVFMNRQFRNCILQLFGKKVDDGSELSSASKTEVSSVSSVSPA

Molecular Weight

43.4 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

OPN1LW Protein, Human (Cell-Free, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
OPN1LW Protein, Human (Cell-Free, His)
Cat. No.:
HY-P702398
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