2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Serine/Threonine Kinase Proteins
  5. MAPK Family
  6. Mitogen-Activated Protein Kinase 13 (p38 delta/MAPK13)
  7. p38 delta/MAPK13 Protein, Human (sf9, GST)

The p38 delta/MAPK13 protein is an important serine/threonine kinase in the MAP kinase pathway. It coordinates cellular responses to stimuli such as cytokines or stress and directly activates the transcription factors ELK1 and ATF2. In this cascade, p38 MAPK (including MAPK13) each phosphorylates approximately 200 to 300 substrates. p38 delta/MAPK13 Protein, Human (sf9, GST) is the recombinant human-derived p38 delta/MAPK13 protein, expressed by Sf9 insect cells , with N-GST labeled tag.

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Description

The p38 delta/MAPK13 protein is an important serine/threonine kinase in the MAP kinase pathway. It coordinates cellular responses to stimuli such as cytokines or stress and directly activates the transcription factors ELK1 and ATF2. In this cascade, p38 MAPK (including MAPK13) each phosphorylates approximately 200 to 300 substrates. p38 delta/MAPK13 Protein, Human (sf9, GST) is the recombinant human-derived p38 delta/MAPK13 protein, expressed by Sf9 insect cells , with N-GST labeled tag.
Background

p38 delta/MAPK13, a serine/threonine kinase and integral component of the MAP kinase signal transduction pathway, is among the four p38 MAPKs pivotal in orchestrating cellular responses triggered by extracellular stimuli like pro-inflammatory cytokines or physical stress, resulting in the direct activation of transcription factors such as ELK1 and ATF2. Operating within this cascade, p38 MAPKs phosphorylate an extensive array of proteins, estimated at approximately 200 to 300 substrates each, with MAPK13 representing one of the less studied isoforms. It targets downstream kinases like MAPKAPK2, activating them through phosphorylation to further impact additional substrates. MAPK13 plays a critical role in regulating protein translation by phosphorylating and inactivating EEF2K, contributing to cytoskeletal remodeling through the phosphorylation of MAPT and STMN1. Moreover, it mediates UV irradiation-induced up-regulation of the gene expression of CXCL14 and plays a vital role in the regulation of epidermal keratinocyte differentiation, apoptosis, and skin tumor development. In response to stress, MAPK13 phosphorylates the transcriptional activator MYB, leading to rapid MYB degradation via a proteasome-dependent pathway. Additionally, MAPK13 phosphorylates and down-regulates PRKD1 during the regulation of insulin secretion in pancreatic beta cells.
Species

Human
Source

Sf9 insect cells

Tag

N-GST
Accession

O15264-1 (M1-L365)
Gene ID
Molecular Construction
N-term
GST
p38δ (M1-L365)
Accession # O15264-1
C-term
Synonyms
Mitogen-activated protein kinase 13; MAPK 13; PRKM13; SAPK4
Molecular Weight

Approximately 63.9 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Data Sheet (235 KB) Handling Instructions (2659 KB)

p38 delta/MAPK13 Protein, Human (sf9, GST) Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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