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  4. Transferases (EC 2)
  5. P38β Protein, Human (Sf9, GST)

The P38β protein is an important serine/threonine kinase in the MAP kinase pathway that responds to extracellular stimuli, including proinflammatory cytokines and physical stress. As part of the p38 MAPK family, P38β directly activates transcription factors that phosphorylate many proteins, including RPS6KA5/MSK1 and RPS6KA4/MSK2. P38β Protein, Human (Sf9, GST) is the recombinant human-derived P38β protein, expressed by Sf9 insect cells , with N-GST labeled tag.

For research use only. We do not sell to patients.

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Description

The P38β protein is an important serine/threonine kinase in the MAP kinase pathway that responds to extracellular stimuli, including proinflammatory cytokines and physical stress. As part of the p38 MAPK family, P38β directly activates transcription factors that phosphorylate many proteins, including RPS6KA5/MSK1 and RPS6KA4/MSK2. P38β Protein, Human (Sf9, GST) is the recombinant human-derived P38β protein, expressed by Sf9 insect cells , with N-GST labeled tag.
Background

P38β, a serine/threonine kinase and an essential component of the MAP kinase signal transduction pathway, operates within the intricate network of cellular responses triggered by extracellular stimuli such as pro-inflammatory cytokines or physical stress. As part of the p38 MAPK family, P38β plays a pivotal role in direct activation of transcription factors, phosphorylating an extensive array of proteins, with an estimated 200 to 300 substrates each. Its functions largely overlap with those of MAPK14, and some of its downstream kinase targets, such as RPS6KA5/MSK1 and RPS6KA4/MSK2, participate in the phosphorylation and activation of transcription factors, chromatin remodeling, and induction of immediate-early genes in response to stress or mitogenic stimuli. Other kinase targets, such as MAPKAPK2/MK2 and MAPKAPK3/MK3, influence gene expression at the post-transcriptional level. In the cytoplasm, P38β regulates protein turnover, exemplified by its phosphorylation of CFLAR, an inhibitor of TNF-induced apoptosis. Furthermore, P38β's involvement in ectodomain shedding of transmembrane proteins, such as ADAM17, highlights its role in cell proliferation and signaling activation. The kinase also phosphorylates NLRP1 downstream of MAP3K20/ZAK, promoting NLRP1 inflammasome activation and pyroptosis in response to UV-B irradiation and ribosome collisions. In the nucleus, P38β emerges as a critical modulator of gene expression by phosphorylating transcription factors and influencing chromatin modifiers and remodelers, thereby regulating processes involved in the inflammatory response.
Species

Human
Source

Sf9 insect cells
Tag

N-GST
Accession

Q15759 (S2-Q364)
Gene ID

5600
Molecular Construction
N-term
GST
P38β (S2-Q364)
Accession # Q15759
C-term
Synonyms
MAPK11; Mitogen-activated protein kinase 11; MAP kinase 11; MAPK 11; Mitogen-activated protein kinase p38 beta; MAP kinase p38 beta; p38b; Stress-activated protein kinase 2b; SAPK2b; p38-2
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Data Sheet (235 KB) Handling Instructions (2659 KB)

P38β Protein, Human (Sf9, GST) Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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P38β Protein, Human (Sf9, GST)
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