1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. PBP1A Protein, Mycobacterium tuberculosis (His)

PBP1A Protein, Mycobacterium tuberculosis (His)

Cat. No.: HY-P701959
Data Sheet Handling Instructions Technical Support

PBP1A (penicillin-binding protein 1A) is involved in cell wall formation and plays a crucial role in the synthesis of cross-linked peptidoglycan from lipid intermediates. The enzyme has a bifunctional structure. The penicillin-insensitive transglycosylase N-terminal domain is responsible for the formation of linear glycan chains, and the penicillin-sensitive transpeptidase C-terminal domain promotes the cross-linking of peptide subunits. PBP1A Protein, Mycobacterium tuberculosis (His) is the recombinant PBP1A protein, expressed by E. coli , with N-6*His labeled tag.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

PBP1A (penicillin-binding protein 1A) is involved in cell wall formation and plays a crucial role in the synthesis of cross-linked peptidoglycan from lipid intermediates. The enzyme has a bifunctional structure. The penicillin-insensitive transglycosylase N-terminal domain is responsible for the formation of linear glycan chains, and the penicillin-sensitive transpeptidase C-terminal domain promotes the cross-linking of peptide subunits. PBP1A Protein, Mycobacterium tuberculosis (His) is the recombinant PBP1A protein, expressed by E. coli , with N-6*His labeled tag.

Background

PBP1A (Penicillin-Binding Protein 1A) is a crucial enzyme involved in cell wall formation in bacteria, specifically in the synthesis of cross-linked peptidoglycan from lipid intermediates. The protein exhibits a bifunctional structure with a penicillin-insensitive transglycosylase N-terminal domain responsible for the formation of linear glycan strands, and a penicillin-sensitive transpeptidase C-terminal domain that facilitates the cross-linking of peptide subunits. While PBP1A has limited peptidoglycan hydrolytic activity on its own, it plays a notable role in inhibiting the synergistic peptidoglycan hydrolysis of RipA plus RpfB.

Species

Others

Source

E. coli

Tag

N-6*His

Accession

P71707 (K391-P820)

Gene ID

887065

Molecular Construction
N-term
6*His
PBP1A (K391-P820)
Accession # P71707
C-term
Synonyms
ponA1; Penicillin-binding protein 1A; PBP-1A; Penicillin-binding protein 1*; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

PBP1A Protein, Mycobacterium tuberculosis (His) Related Classifications

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
PBP1A Protein, Mycobacterium tuberculosis (His)
Cat. No.:
HY-P701959
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