PCBP1 Protein, Human (His)

PCBP1, a single-stranded nucleic acid binding protein with a specific affinity for oligo dC, is crucial for erythropoiesis when paired with PCBP2, potentially impacting mRNA splicing. In poliovirus infection, PCBP1 plays a vital role in initiating viral RNA replication alongside the viral protein 3CD, showcasing its multifunctional capacity in nucleic acid binding and involvement in fundamental cellular and viral processes. PCBP1 Protein, Human (His) is the recombinant human-derived PCBP1 protein, expressed by E. coli , with N-His labeled tag. The total length of PCBP1 Protein, Human (His) is 355 a.a., with molecular weight of ~42 kDa.

PCBP1 is a single-stranded nucleic acid binding protein with a specific affinity for oligo dC. Paired with PCBP2, it is essential for erythropoiesis, potentially influencing mRNA splicing processes. In the context of poliovirus infection, PCBP1 assumes a crucial role in initiating viral RNA replication in collaboration with the viral protein 3CD. This highlights its multifunctional capacity in nucleic acid binding and its involvement in fundamental cellular and viral processes.

Human

E. coli

N-His

Q15365 (D2-C356)

5093  [NCBI]

DAGVTESGLNVTLTIRLLMHGKEVGSIIGKKGESVKRIREESGARINISEGNCPERIITLTGPTNAIFKAFAMIIDKLEEDINSSMTNSTAASRPPVTLRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDRCSDAAGYPHATHDLEGPPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAMMHGGTGFAGIDSSSPEVKGYWASLDASTQTTHELTIPNNLIGCIIGRQGANINEIRQMSGAQIKIANPVEGSSGRQVTITGSAASISLAQYLINARLSSEKGMGC

Approximately 42 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 500 mM NaCl, 20% glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.