PDGF R alpha Protein, Mouse (HEK293, His)

PDGF R α is a tyrosine-protein kinase receptor with critical effects on embryonic development, cell proliferation, survival, and chemotaxis.Its effects on cell behavior vary depending on the environment and is critical for differentiation of bone marrow mesenchymal stem cells, normal skeletal development, embryonic skull closure, and gastrointestinal mucosal formation.PDGF R alpha Protein, Mouse (HEK293, His) is the recombinant mouse-derived PDGF R alpha protein, expressed by HEK293 , with C-6*His labeled tag.

The PDGF R alpha protein is a tyrosine-protein kinase that functions as a cell-surface receptor for PDGFA, PDGFB, and PDGFC. It plays a crucial role in embryonic development, cell proliferation, survival, and chemotaxis. Its effects on cell proliferation and migration vary depending on the context. The protein is also involved in the differentiation of bone marrow-derived mesenchymal stem cells and is required for normal skeleton development and cephalic closure during embryonic development. Additionally, it is essential for the development of the gastrointestinal tract lining, recruitment of mesenchymal cells, and normal development of intestinal villi. The protein contributes to cell migration and chemotaxis in wound healing and plays a role in platelet activation, secretion of agonists from platelet granules, and thrombin-induced platelet aggregation. Activation of the protein occurs upon binding to its ligands, which can be homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB, or homodimeric PDGFC. This activation leads to the activation of multiple signaling cascades, with the response depending on the specific ligand and being modulated by the formation of heterodimers between PDGFRA and PDGFRB. The protein phosphorylates PIK3R1, PLCG1, and PTPN11, resulting in various cellular responses such as the production of diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+), activation of protein kinase C, and activation of the AKT1 signaling pathway. It also mediates the activation of HRAS and MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Furthermore, it promotes the activation of STAT family members STAT1, STAT3, and STAT5A and/or STAT5B. The receptor signaling is regulated by protein phosphatases that dephosphorylate the receptor and its downstream effectors, as well as by rapid internalization of the activated receptor.

Mouse

HEK293

C-6*His

P26618 (L25-E524)

18595  [NCBI]

LLLPSILPNENEKIVQLNSSFSLRCVGESEVSWQHPMSEEDDPNVEIRSEENNSGLFVTVLEVVNASAAHTGWYTCYYNHTQTDESEIEGRHIYIYVPDPDMAFVPLGMTDSLVIVEEDDSAIIPCRTTDPETQVTLHNNGRLVPASYDSRQGFNGTFSVGPYICEATVKGRTFKTSEFNVYALKATSELNLEMDARQTVYKAGETIVVTCAVFNNEVVDLQWTYPGEVRNKGITMLEEIKLPSIKLVYTLTVPKATVKDSGEYECAARQATKEVKEMKRVTISVHEKGFVEIEPTFGQLEAVNLHEVREFVVEVQAYPTPRISWLKDNLTLIENLTEITTDVQKSQETRYQSKLKLIRAKEEDSGHYTIIVQNEDDVKSYTFELSTLVPASILDLVDDHHGSGGGQTVRCTAEGTPLPEIDWMICKHIKKCNNDTSWTVLASNVSNIITELPRRGRSTVEGRVSFAKVEETIAVRCLAKNNLSVVARELKLVAPTLRSE

80-120 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.